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miRNA: 3'- -CUACuaGaaGUUG--------AGGAGGUCCGCGU- -5'
miRNA: 3'- -CUACUAGaAGUUGag-GAGGUCCgCGu -5'
miRNA: 3'- cUACUAGaaGUUGaggAGG-UCCGCGU- -5'
miRNA: 3'- cuACUAGAAGUUGA-GGAgGU-CCGcGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 16098 | 5' | -52.4 | NC_004065.1 | + | 208883 | 0.66 | 0.990723 |
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Target: 5'- cGGUGcgCggUGACggacugagUUCUCCGGGCGCAg -3' miRNA: 3'- -CUACuaGaaGUUG--------AGGAGGUCCGCGU- -5' |
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| 16098 | 5' | -52.4 | NC_004065.1 | + | 136378 | 0.66 | 0.989439 |
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Target: 5'- aGAUGGUCaUCGAUggaCUCCAGGaGCc -3' miRNA: 3'- -CUACUAGaAGUUGag-GAGGUCCgCGu -5' |
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| 16098 | 5' | -52.4 | NC_004065.1 | + | 104211 | 0.66 | 0.988021 |
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Target: 5'- cGUGAUCagCGACggaUCCgAGGCGCAc -3' miRNA: 3'- cUACUAGaaGUUGaggAGG-UCCGCGU- -5' |
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| 16098 | 5' | -52.4 | NC_004065.1 | + | 132632 | 0.66 | 0.993852 |
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Target: 5'- -cUGAUCUUCGACUaCCUgCGcGGC-CAc -3' miRNA: 3'- cuACUAGAAGUUGA-GGAgGU-CCGcGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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