Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
16098 | 5' | -52.4 | NC_004065.1 | + | 87629 | 0.66 | 0.992921 |
Target: 5'- --cGAUCgUCAGCgCCagcUCGGGCGCAa -3' miRNA: 3'- cuaCUAGaAGUUGaGGa--GGUCCGCGU- -5' |
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16098 | 5' | -52.4 | NC_004065.1 | + | 210130 | 0.66 | 0.992921 |
Target: 5'- ---cGUCUUCGAUUCCUCgGcGGCGaCGg -3' miRNA: 3'- cuacUAGAAGUUGAGGAGgU-CCGC-GU- -5' |
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16098 | 5' | -52.4 | NC_004065.1 | + | 118752 | 0.66 | 0.993852 |
Target: 5'- aGAUGGUCUUCu-CUCggUCAcGGCGCc -3' miRNA: 3'- -CUACUAGAAGuuGAGgaGGU-CCGCGu -5' |
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16098 | 5' | -52.4 | NC_004065.1 | + | 132632 | 0.66 | 0.993852 |
Target: 5'- -cUGAUCUUCGACUaCCUgCGcGGC-CAc -3' miRNA: 3'- cuACUAGAAGUUGA-GGAgGU-CCGcGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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