miRNA: 3'- cuaCUaGAaGUUG----AGGAGGUCCGCGu -5'
miRNA: 3'- cuaCUAGAAGUUG--AGG-------------AGGU-CCGCGu -5'
miRNA: 3'- cUACUAGaaGUUGaggAGG-UCCGCGU- -5'
miRNA: 3'- -CUACUAGAAGUUGAGGAGGUCCGCGU- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16098 | 5' | -52.4 | NC_004065.1 | + | 109658 | 0.67 | 0.984746 |
|
Target: 5'- --cGA-CUcCGGCagggUCCUCCGGGCGCu -3' miRNA: 3'- cuaCUaGAaGUUG----AGGAGGUCCGCGu -5' |
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| 16098 | 5' | -52.4 | NC_004065.1 | + | 201513 | 0.67 | 0.986295 |
|
Target: 5'- --aGAUCUUCAGCagUCCcaugcgacggagcUCCGcGGCGCu -3' miRNA: 3'- cuaCUAGAAGUUG--AGG-------------AGGU-CCGCGu -5' |
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| 16098 | 5' | -52.4 | NC_004065.1 | + | 104211 | 0.66 | 0.988021 |
|
Target: 5'- cGUGAUCagCGACggaUCCgAGGCGCAc -3' miRNA: 3'- cUACUAGaaGUUGaggAGG-UCCGCGU- -5' |
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| 16098 | 5' | -52.4 | NC_004065.1 | + | 113170 | 1.08 | 0.009134 |
|
Target: 5'- uGAUGAUCUUCAACUCCUCCAGGCGCAu -3' miRNA: 3'- -CUACUAGAAGUUGAGGAGGUCCGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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