Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
161 | 5' | -56.7 | AC_000007.1 | + | 9243 | 0.66 | 0.520271 |
Target: 5'- gCCAugGAGCGCCUugAgGCcuuGGGGGa- -3' miRNA: 3'- -GGUugCUCGCGGA--UgCGcc-UCUCCau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 23760 | 0.67 | 0.46766 |
Target: 5'- -uGGCGGGCGCUcGgGCuugGGAGAGGg- -3' miRNA: 3'- ggUUGCUCGCGGaUgCG---CCUCUCCau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 11405 | 0.67 | 0.46766 |
Target: 5'- aCC-GCGAGCGguugCUGCGCGaGGAGGa- -3' miRNA: 3'- -GGuUGCUCGCg---GAUGCGCcUCUCCau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 5093 | 0.67 | 0.437439 |
Target: 5'- gCGGCGAGCGCCUucugGCGCauuuGGucAGGg- -3' miRNA: 3'- gGUUGCUCGCGGA----UGCG----CCucUCCau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 34205 | 0.68 | 0.380537 |
Target: 5'- gCCAGgGGGCGCCUggAUGCuuuGAGAGaGUGg -3' miRNA: 3'- -GGUUgCUCGCGGA--UGCGc--CUCUC-CAU- -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 11220 | 0.69 | 0.354002 |
Target: 5'- -gGGCGAGgGCCUgGCGCGGcuAGGa- -3' miRNA: 3'- ggUUGCUCgCGGA-UGCGCCucUCCau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 8959 | 0.71 | 0.254521 |
Target: 5'- aCCGugGAGCgaGCCggACGCGGAGAu--- -3' miRNA: 3'- -GGUugCUCG--CGGa-UGCGCCUCUccau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 26507 | 0.71 | 0.254521 |
Target: 5'- gCCGGCG-GCGCCUGaggaGCGGAGGuuGUAg -3' miRNA: 3'- -GGUUGCuCGCGGAUg---CGCCUCUc-CAU- -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 16853 | 0.73 | 0.184189 |
Target: 5'- gCCAACGAGCGCCU-CGgGGAGuuugccuacGGa- -3' miRNA: 3'- -GGUUGCUCGCGGAuGCgCCUCu--------CCau -5' |
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161 | 5' | -56.7 | AC_000007.1 | + | 6511 | 1.09 | 0.000412 |
Target: 5'- aCCAACGAGCGCCUACGCGGAGAGGUAg -3' miRNA: 3'- -GGUUGCUCGCGGAUGCGCCUCUCCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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