Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1611 | 5' | -50.7 | NC_001347.2 | + | 166828 | 0.68 | 0.993632 |
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Target: 5'- -aGGUGCUCga-GAGUCGUgGCGAUg -3' miRNA: 3'- gaCCACGAGgcaCUUAGCAaUGCUGa -5' |
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| 1611 | 5' | -50.7 | NC_001347.2 | + | 105623 | 0.68 | 0.993632 |
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Target: 5'- gCUGGUGCUgCCcUGGAUCGUacccaUGCcACUg -3' miRNA: 3'- -GACCACGA-GGcACUUAGCA-----AUGcUGA- -5' |
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| 1611 | 5' | -50.7 | NC_001347.2 | + | 12213 | 0.73 | 0.924491 |
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Target: 5'- uCUGGUGCUCCuGUuaGGA-CGUUACGAa- -3' miRNA: 3'- -GACCACGAGG-CA--CUUaGCAAUGCUga -5' |
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| 1611 | 5' | -50.7 | NC_001347.2 | + | 187347 | 0.73 | 0.918912 |
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Target: 5'- -aGGUGCguugcguggCCGUGGcgCGUcGCGGCUa -3' miRNA: 3'- gaCCACGa--------GGCACUuaGCAaUGCUGA- -5' |
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| 1611 | 5' | -50.7 | NC_001347.2 | + | 174580 | 0.74 | 0.894201 |
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Target: 5'- gUGG-GCaCUGUGAGUCGUUGCGAa- -3' miRNA: 3'- gACCaCGaGGCACUUAGCAAUGCUga -5' |
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| 1611 | 5' | -50.7 | NC_001347.2 | + | 149337 | 1.09 | 0.014895 |
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Target: 5'- gCUGGUGCUCCGUGAAUCGUUACGACUg -3' miRNA: 3'- -GACCACGAGGCACUUAGCAAUGCUGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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