Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16112 | 3' | -58.1 | NC_004065.1 | + | 133787 | 0.67 | 0.819582 |
Target: 5'- -uGCGGGUCUUGgAUCuCGCGGuGUCCu -3' miRNA: 3'- cuUGUCCAGGACgUAG-GCGCU-CGGGu -5' |
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16112 | 3' | -58.1 | NC_004065.1 | + | 228459 | 0.68 | 0.811175 |
Target: 5'- cGAACGGGaCC-GCGUugcucaUCGCGAGUCCGg -3' miRNA: 3'- -CUUGUCCaGGaCGUA------GGCGCUCGGGU- -5' |
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16112 | 3' | -58.1 | NC_004065.1 | + | 123842 | 1.08 | 0.003202 |
Target: 5'- gGAACAGGUCCUGCAUCCGCGAGCCCAu -3' miRNA: 3'- -CUUGUCCAGGACGUAGGCGCUCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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