miRNA: 3'- gCGUGCGCGGCGuCCACGcgGcaCGU- -5'
miRNA: 3'- gCGUGc----GCGGCG--UCCaCGCGGCa-CGU- -5'
Results 61 - 62 of 62 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16112 | 5' | -63.1 | NC_004065.1 | + | 219253 | 0.67 | 0.628056 |
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Target: 5'- aGCACGCGCCGCcGGUGUaaCucGCGa -3' miRNA: 3'- gCGUGCGCGGCGuCCACGcgGcaCGU- -5' |
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| 16112 | 5' | -63.1 | NC_004065.1 | + | 229940 | 0.66 | 0.703932 |
|
Target: 5'- uGCACuuuuuUGCCGCcgAGG-GCGCCGcgGCAc -3' miRNA: 3'- gCGUGc----GCGGCG--UCCaCGCGGCa-CGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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