Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1612 | 3' | -57.4 | NC_001347.2 | + | 128665 | 0.66 | 0.934938 |
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Target: 5'- -aCGCGcccgugccGCCGGcGACCGCCGUCu -3' miRNA: 3'- caGCGUuucc----UGGCC-CUGGUGGCAGc -5' |
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| 1612 | 3' | -57.4 | NC_001347.2 | + | 135806 | 0.66 | 0.934938 |
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Target: 5'- -cCGCGGcggguGGACCGGGAa-GCCGgCGg -3' miRNA: 3'- caGCGUUu----CCUGGCCCUggUGGCaGC- -5' |
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| 1612 | 3' | -57.4 | NC_001347.2 | + | 82769 | 0.66 | 0.939554 |
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Target: 5'- aGUCGCGAAGGcCCGcGGGgucuuuuuUCACCGa-- -3' miRNA: 3'- -CAGCGUUUCCuGGC-CCU--------GGUGGCagc -5' |
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| 1612 | 3' | -57.4 | NC_001347.2 | + | 143491 | 0.66 | 0.939554 |
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Target: 5'- cUCGCGccagccGGACCGGGcgGCCuucuaccguACCGUCc -3' miRNA: 3'- cAGCGUuu----CCUGGCCC--UGG---------UGGCAGc -5' |
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| 1612 | 3' | -57.4 | NC_001347.2 | + | 34291 | 0.66 | 0.943954 |
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Target: 5'- -cCGCAuGGGAaaguaCGGuGucGCCACCGUUGa -3' miRNA: 3'- caGCGUuUCCUg----GCC-C--UGGUGGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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