Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1613 | 3' | -53.9 | NC_001347.2 | + | 220672 | 0.66 | 0.989615 |
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Target: 5'- -gUUCCgUAUCUaGGUccGUCCGCaGAUGu -3' miRNA: 3'- gaAAGGgAUAGA-CCA--CAGGCGgCUGC- -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 130752 | 0.66 | 0.989615 |
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Target: 5'- ---cCCCUcgCcGGUGUCCGgagucaCCGugGu -3' miRNA: 3'- gaaaGGGAuaGaCCACAGGC------GGCugC- -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 206183 | 0.66 | 0.985131 |
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Target: 5'- uCUUUCCCcGUCcagguaGGUGggguaggCCGCCGAg- -3' miRNA: 3'- -GAAAGGGaUAGa-----CCACa------GGCGGCUgc -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 129881 | 0.67 | 0.979302 |
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Target: 5'- --aUCCCUAUCUGGaGaCgGUgGGCGg -3' miRNA: 3'- gaaAGGGAUAGACCaCaGgCGgCUGC- -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 15800 | 0.68 | 0.96575 |
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Target: 5'- uCUUUCUCUGcuUCUuuucuggGGUGUCUaGCUGGCGg -3' miRNA: 3'- -GAAAGGGAU--AGA-------CCACAGG-CGGCUGC- -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 53060 | 0.71 | 0.880896 |
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Target: 5'- ---gCCCUGUCUGGcGUCUccgugGUCGACGc -3' miRNA: 3'- gaaaGGGAUAGACCaCAGG-----CGGCUGC- -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 195666 | 0.73 | 0.802049 |
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Target: 5'- ---gCCCgaggAUCUGGcGgCCGCCGGCGg -3' miRNA: 3'- gaaaGGGa---UAGACCaCaGGCGGCUGC- -5' |
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| 1613 | 3' | -53.9 | NC_001347.2 | + | 148070 | 1.1 | 0.006892 |
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Target: 5'- uCUUUCCCUAUCUGGUGUCCGCCGACGg -3' miRNA: 3'- -GAAAGGGAUAGACCACAGGCGGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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