Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16139 | 3' | -55.6 | NC_004065.1 | + | 204700 | 0.66 | 0.96452 |
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Target: 5'- gACUGCCgcaggacccugGGAuCGCUGUGuUCGUGGgCUUg -3' miRNA: 3'- -UGAUGG-----------CCU-GUGACGC-AGCACCaGAA- -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 11984 | 0.66 | 0.96452 |
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Target: 5'- -aUGCUGGugGCcGCGaUCGUcgcGGUCUUa -3' miRNA: 3'- ugAUGGCCugUGaCGC-AGCA---CCAGAA- -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 82617 | 0.66 | 0.953851 |
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Target: 5'- uGCUGCUGGACGCgguUGCGUCcggGGg--- -3' miRNA: 3'- -UGAUGGCCUGUG---ACGCAGca-CCagaa -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 139728 | 0.67 | 0.92492 |
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Target: 5'- gGCUGCCGGGCAUccccaagcaGaucaagaaggagccCGUCGUGGUCa- -3' miRNA: 3'- -UGAUGGCCUGUGa--------C--------------GCAGCACCAGaa -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 165886 | 0.67 | 0.915552 |
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Target: 5'- gGCgagGCCGcacGCGCUGcCGUCGUGGUa-- -3' miRNA: 3'- -UGa--UGGCc--UGUGAC-GCAGCACCAgaa -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 203019 | 0.68 | 0.89091 |
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Target: 5'- gUUACCGGACGgUaGCGggUCGUGGUgUUg -3' miRNA: 3'- uGAUGGCCUGUgA-CGC--AGCACCAgAA- -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 79622 | 0.68 | 0.89091 |
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Target: 5'- cGCUGCgcgcgCGGACGCUGCGggCGUGcguGUCg- -3' miRNA: 3'- -UGAUG-----GCCUGUGACGCa-GCAC---CAGaa -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 88449 | 0.72 | 0.693152 |
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Target: 5'- -gUGCCGGGCGaggGCGUCGcaGGUCUUg -3' miRNA: 3'- ugAUGGCCUGUga-CGCAGCa-CCAGAA- -5' |
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| 16139 | 3' | -55.6 | NC_004065.1 | + | 141277 | 1.04 | 0.009306 |
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Target: 5'- uACUACCGGACACUGCGUCGUGGUCUUc -3' miRNA: 3'- -UGAUGGCCUGUGACGCAGCACCAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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