Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1614 | 5' | -56 | NC_001347.2 | + | 1604 | 0.66 | 0.954509 |
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Target: 5'- gGUGGUgcggcuguaccgcUGCAacCGGCUGGGCGUgGGCa -3' miRNA: 3'- -CGCCA-------------AUGUa-GUUGGCCCGCGaCCGc -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 59041 | 0.66 | 0.958549 |
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Target: 5'- gGCGGaggaGCAUCcguGACCucgagccaccauGGGCGC-GGCGg -3' miRNA: 3'- -CGCCaa--UGUAG---UUGG------------CCCGCGaCCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 184967 | 0.66 | 0.962002 |
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Target: 5'- gGCGGcucGCGgaGACUGGGCuGCaGGCGg -3' miRNA: 3'- -CGCCaa-UGUagUUGGCCCG-CGaCCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 1735 | 0.66 | 0.964296 |
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Target: 5'- gGCGGUgggccgGCA-CGACgGuGGCGCUgcagacgccgggcaGGCGa -3' miRNA: 3'- -CGCCAa-----UGUaGUUGgC-CCGCGA--------------CCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 144578 | 0.66 | 0.964934 |
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Target: 5'- aCGGUgGCggCGGCCGGGCucguggaGgaGGCa -3' miRNA: 3'- cGCCAaUGuaGUUGGCCCG-------CgaCCGc -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 9181 | 0.66 | 0.964934 |
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Target: 5'- uGUGGUaccaggaUACAUCG--CGGGaaaagaguCGCUGGCGa -3' miRNA: 3'- -CGCCA-------AUGUAGUugGCCC--------GCGACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 55358 | 0.66 | 0.965249 |
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Target: 5'- uGCGGUuuuggcagUACAUCAA-UGGGCGUggauaGCGg -3' miRNA: 3'- -CGCCA--------AUGUAGUUgGCCCGCGac---CGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 142566 | 0.66 | 0.965249 |
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Target: 5'- gGCuGGcgGCGUCGGCgGGaGCaGCgggGGCGg -3' miRNA: 3'- -CG-CCaaUGUAGUUGgCC-CG-CGa--CCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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