Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1614 | 5' | -56 | NC_001347.2 | + | 191782 | 0.67 | 0.938079 |
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Target: 5'- uGUGGcgGCA-CAGCCGcaGGCGCUcugccaguuGGCGa -3' miRNA: 3'- -CGCCaaUGUaGUUGGC--CCGCGA---------CCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 192167 | 0.68 | 0.893786 |
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Target: 5'- cGCGGUUuccgcCGUCuccggaugagcGGCCGcGGCGCgGGCu -3' miRNA: 3'- -CGCCAAu----GUAG-----------UUGGC-CCGCGaCCGc -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 193966 | 0.67 | 0.927836 |
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Target: 5'- uGCGGcgACA-CGggcACCGuguacgcGGCGCUGGUGg -3' miRNA: 3'- -CGCCaaUGUaGU---UGGC-------CCGCGACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 197284 | 0.73 | 0.661429 |
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Target: 5'- cGUGGgguCAUCGACCGacaaGGCGC-GGCGa -3' miRNA: 3'- -CGCCaauGUAGUUGGC----CCGCGaCCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 209056 | 0.68 | 0.917715 |
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Target: 5'- cGCGGUUGCgGUCGcgcACCGGcUGCagacagGGCGa -3' miRNA: 3'- -CGCCAAUG-UAGU---UGGCCcGCGa-----CCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 222660 | 0.73 | 0.641628 |
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Target: 5'- cGCGaGUguucCAUaaaAGCCGGGCGCUccGGCGa -3' miRNA: 3'- -CGC-CAau--GUAg--UUGGCCCGCGA--CCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 229621 | 0.67 | 0.938079 |
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Target: 5'- cGCGG--GCGUguGCCGGGUGUgucgugccgGGUGu -3' miRNA: 3'- -CGCCaaUGUAguUGGCCCGCGa--------CCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 229668 | 0.67 | 0.933324 |
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Target: 5'- gGCGGgugUGC--CGGCgGGGUGUggUGGCGg -3' miRNA: 3'- -CGCCa--AUGuaGUUGgCCCGCG--ACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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