Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1614 | 5' | -56 | NC_001347.2 | + | 182400 | 0.67 | 0.923143 |
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Target: 5'- -aGGUgUACGUgGACgCGGGCG-UGGUGg -3' miRNA: 3'- cgCCA-AUGUAgUUG-GCCCGCgACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 30597 | 0.67 | 0.923143 |
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Target: 5'- gGCGGcuuccUGCGgcCGGCCGcGGUGCcGGCGg -3' miRNA: 3'- -CGCCa----AUGUa-GUUGGC-CCGCGaCCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 75404 | 0.67 | 0.928346 |
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Target: 5'- aGCGGgaGCggCGGCCGuGGCgGC-GGCa -3' miRNA: 3'- -CGCCaaUGuaGUUGGC-CCG-CGaCCGc -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 193966 | 0.67 | 0.927836 |
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Target: 5'- uGCGGcgACA-CGggcACCGuguacgcGGCGCUGGUGg -3' miRNA: 3'- -CGCCaaUGUaGU---UGGC-------CCGCGACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 189 | 0.67 | 0.933324 |
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Target: 5'- gGCGGgugUGC--CGGCgGGGUGUggUGGCGg -3' miRNA: 3'- -CGCCa--AUGuaGUUGgCCCGCG--ACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 8603 | 0.67 | 0.928346 |
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Target: 5'- -gGGUgccCGUCAcGCa-GGCGCUGGCGu -3' miRNA: 3'- cgCCAau-GUAGU-UGgcCCGCGACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 229668 | 0.67 | 0.933324 |
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Target: 5'- gGCGGgugUGC--CGGCgGGGUGUggUGGCGg -3' miRNA: 3'- -CGCCa--AUGuaGUUGgCCCGCG--ACCGC- -5' |
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| 1614 | 5' | -56 | NC_001347.2 | + | 55358 | 0.66 | 0.965249 |
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Target: 5'- uGCGGUuuuggcagUACAUCAA-UGGGCGUggauaGCGg -3' miRNA: 3'- -CGCCA--------AUGUAGUUgGCCCGCGac---CGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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