Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1615 | 5' | -60.9 | NC_001347.2 | + | 168736 | 0.66 | 0.769923 |
Target: 5'- gUCCGUGGCCcguGCCCgcagCUGCUCUa-- -3' miRNA: 3'- -GGGCACCGGuu-CGGGa---GGUGGGAcug -5' |
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1615 | 5' | -60.9 | NC_001347.2 | + | 87584 | 0.67 | 0.75561 |
Target: 5'- gCCCGUGGCCGugaGcagaccgcggugcacGCCCgugcCCACCagcGACg -3' miRNA: 3'- -GGGCACCGGU---U---------------CGGGa---GGUGGga-CUG- -5' |
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1615 | 5' | -60.9 | NC_001347.2 | + | 189988 | 0.82 | 0.114663 |
Target: 5'- gCCUGUGGCCuccCCCUCCAUCCUGAa -3' miRNA: 3'- -GGGCACCGGuucGGGAGGUGGGACUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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