Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16161 | 3' | -55.9 | NC_004065.1 | + | 144236 | 0.7 | 0.843386 |
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Target: 5'- cGCCGGUU-CUCGcgcCUCG-GUCGCCGa -3' miRNA: 3'- cUGGCCAGuGAGCa--GAGCuCAGUGGC- -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 141108 | 0.71 | 0.819319 |
|
Target: 5'- cGGCCGcUCGCUCGUCUCcgucAGcuUCGCCGu -3' miRNA: 3'- -CUGGCcAGUGAGCAGAGc---UC--AGUGGC- -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 71502 | 0.71 | 0.810973 |
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Target: 5'- cGCCGGUCGCg---CUCGcAGUCugCGa -3' miRNA: 3'- cUGGCCAGUGagcaGAGC-UCAGugGC- -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 162303 | 0.71 | 0.810974 |
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Target: 5'- cGAgCGGUCGCUCGUCacggCGA--UACCGa -3' miRNA: 3'- -CUgGCCAGUGAGCAGa---GCUcaGUGGC- -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 31251 | 0.71 | 0.810974 |
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Target: 5'- cGACCGuGUCcgaUCGUCUCugcGGUCGCCa -3' miRNA: 3'- -CUGGC-CAGug-AGCAGAGc--UCAGUGGc -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 223478 | 0.71 | 0.802478 |
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Target: 5'- cACCGGUCccgagAC-CGUCUCGGGaccagcagaUCGCCGg -3' miRNA: 3'- cUGGCCAG-----UGaGCAGAGCUC---------AGUGGC- -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 223942 | 0.71 | 0.79384 |
|
Target: 5'- aGACCGGgu-CUCGUCagcggcaGGGUCACCa -3' miRNA: 3'- -CUGGCCaguGAGCAGag-----CUCAGUGGc -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 191080 | 0.73 | 0.672195 |
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Target: 5'- cGCCGGUCGCUCGagCUCGA--CACCc -3' miRNA: 3'- cUGGCCAGUGAGCa-GAGCUcaGUGGc -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 68076 | 0.75 | 0.60343 |
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Target: 5'- cGAgCGGUCACUCGaCgCGAG-CACCGa -3' miRNA: 3'- -CUgGCCAGUGAGCaGaGCUCaGUGGC- -5' |
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| 16161 | 3' | -55.9 | NC_004065.1 | + | 161909 | 1.08 | 0.005881 |
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Target: 5'- cGACCGGUCACUCGUCUCGAGUCACCGu -3' miRNA: 3'- -CUGGCCAGUGAGCAGAGCUCAGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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