Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16176 | 3' | -44.7 | NC_004065.1 | + | 222070 | 0.66 | 1 |
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Target: 5'- --gUGUGGUGGgaAUCGgcacGggUuUCGUGg -3' miRNA: 3'- gaaACACCACU--UAGCa---CuuAuAGCGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 1831 | 0.66 | 1 |
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Target: 5'- ---cGUGGUGAcggugAUCGUGuugGUGUC-CGg -3' miRNA: 3'- gaaaCACCACU-----UAGCACu--UAUAGcGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 167298 | 0.67 | 1 |
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Target: 5'- --aUGUGGUGA-UgGUGGGauUCGUGg -3' miRNA: 3'- gaaACACCACUuAgCACUUauAGCGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 110688 | 0.67 | 0.999999 |
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Target: 5'- --cUGUGGUGAGUgugaCGUGAGccgccgcCGCGa -3' miRNA: 3'- gaaACACCACUUA----GCACUUaua----GCGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 36150 | 0.68 | 0.999998 |
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Target: 5'- --gUGuUGGUGGAUCGgcggGAGUcgCcGCGg -3' miRNA: 3'- gaaAC-ACCACUUAGCa---CUUAuaG-CGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 82340 | 0.68 | 0.999997 |
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Target: 5'- ---gGUGGUG--UCGcUGAcgAUCGCGu -3' miRNA: 3'- gaaaCACCACuuAGC-ACUuaUAGCGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 85351 | 0.69 | 0.999982 |
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Target: 5'- cCUUcGUGGUGAcgcUCGUGAcccacuacgaGUcgCGCGa -3' miRNA: 3'- -GAAaCACCACUu--AGCACU----------UAuaGCGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 203318 | 0.72 | 0.999549 |
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Target: 5'- ---aGUaGGUGggUCGggGAuUAUCGCGu -3' miRNA: 3'- gaaaCA-CCACuuAGCa-CUuAUAGCGC- -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 138056 | 0.76 | 0.989475 |
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Target: 5'- ---cGUGGUGAucacgGUCGUGGgcuuGUGUCGCu -3' miRNA: 3'- gaaaCACCACU-----UAGCACU----UAUAGCGc -5' |
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| 16176 | 3' | -44.7 | NC_004065.1 | + | 174563 | 1.12 | 0.039384 |
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Target: 5'- cCUUUGUGGUGAAUCGUGAAUAUCGCGg -3' miRNA: 3'- -GAAACACCACUUAGCACUUAUAGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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