Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1618 | 3' | -55.6 | NC_001347.2 | + | 1853 | 0.66 | 0.977419 |
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Target: 5'- ----cCCCGCGUCGcugcUGACGgccguGCGUCGg -3' miRNA: 3'- gucuuGGGCGCAGCa---GCUGC-----UGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 110510 | 0.66 | 0.977419 |
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Target: 5'- uCAGAGCCaGCGgCG-CGGCGGCGa-- -3' miRNA: 3'- -GUCUUGGgCGCaGCaGCUGCUGCagc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 151527 | 0.66 | 0.977419 |
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Target: 5'- aAGAcgGCCUGCGaguUCGUCaAgGGCGUCa -3' miRNA: 3'- gUCU--UGGGCGC---AGCAGcUgCUGCAGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 99562 | 0.66 | 0.977419 |
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Target: 5'- aCAGcuuguGACCCGCGcUCGUCGuCGggaGCG-CGg -3' miRNA: 3'- -GUC-----UUGGGCGC-AGCAGCuGC---UGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 85572 | 0.66 | 0.979607 |
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Target: 5'- gCAGaAACUCGCGU-GcCGACGGgGUCu -3' miRNA: 3'- -GUC-UUGGGCGCAgCaGCUGCUgCAGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 158696 | 0.66 | 0.979607 |
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Target: 5'- aCAGAccGCCUGCcaCGUCGGCGGCc--- -3' miRNA: 3'- -GUCU--UGGGCGcaGCAGCUGCUGcagc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 12792 | 0.66 | 0.979607 |
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Target: 5'- cCAGGccgGCCgGCGUuuucacuacgcCGUgGACGACGcCGa -3' miRNA: 3'- -GUCU---UGGgCGCA-----------GCAgCUGCUGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 95099 | 0.66 | 0.981635 |
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Target: 5'- aCAGGcGCCCGaggCGUCGcacAUGACGUCu -3' miRNA: 3'- -GUCU-UGGGCgcaGCAGC---UGCUGCAGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 34180 | 0.66 | 0.981635 |
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Target: 5'- --uAAUCCGCaccuGUCGUCGAC-AUGUCGc -3' miRNA: 3'- gucUUGGGCG----CAGCAGCUGcUGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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