Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1618 | 3' | -55.6 | NC_001347.2 | + | 166828 | 0.67 | 0.957059 |
|
Target: 5'- aGGuGCUCGagaGUCGU-GGCGAUGUCGa -3' miRNA: 3'- gUCuUGGGCg--CAGCAgCUGCUGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 182112 | 0.71 | 0.827201 |
|
Target: 5'- cCAGAucguCCCGCGg-GUCuuCGACGUCGc -3' miRNA: 3'- -GUCUu---GGGCGCagCAGcuGCUGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 182214 | 0.66 | 0.969818 |
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Target: 5'- gCGGG--CUGCGUCGccuUCGGUGACGUCGg -3' miRNA: 3'- -GUCUugGGCGCAGC---AGCUGCUGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 182508 | 0.68 | 0.936499 |
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Target: 5'- aAGAAgCCGCGUCGgUCacagGACGGCGa-- -3' miRNA: 3'- gUCUUgGGCGCAGC-AG----CUGCUGCagc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 189344 | 0.66 | 0.975061 |
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Target: 5'- uCAGAcGCCCGagcaGUCGACGcCGUCc -3' miRNA: 3'- -GUCU-UGGGCgcagCAGCUGCuGCAGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 192291 | 0.67 | 0.957059 |
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Target: 5'- cCGGGcCCCGCGUUcaaGACGGCGUg- -3' miRNA: 3'- -GUCUuGGGCGCAGcagCUGCUGCAgc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 193171 | 0.67 | 0.953368 |
|
Target: 5'- -uGGGCaCGCG-CGUCGGCcGCGUCGc -3' miRNA: 3'- guCUUGgGCGCaGCAGCUGcUGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 196572 | 0.66 | 0.969818 |
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Target: 5'- --cAACCCGCGUCG-CGGCuACGcCa -3' miRNA: 3'- gucUUGGGCGCAGCaGCUGcUGCaGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 208117 | 0.71 | 0.835172 |
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Target: 5'- gCGGAG-CCGCGUCGcUCGcCGGCGcUCGu -3' miRNA: 3'- -GUCUUgGGCGCAGC-AGCuGCUGC-AGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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