Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1618 | 3' | -55.6 | NC_001347.2 | + | 34180 | 0.66 | 0.981635 |
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Target: 5'- --uAAUCCGCaccuGUCGUCGAC-AUGUCGc -3' miRNA: 3'- gucUUGGGCG----CAGCAGCUGcUGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 31584 | 0.69 | 0.921582 |
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Target: 5'- uCAGGccAgCUGCGUCGUCaagGACGGCGUg- -3' miRNA: 3'- -GUCU--UgGGCGCAGCAG---CUGCUGCAgc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 26096 | 0.69 | 0.916175 |
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Target: 5'- gGGAAaCCGCGUCGUCGGaaACGgagCGu -3' miRNA: 3'- gUCUUgGGCGCAGCAGCUgcUGCa--GC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 25131 | 0.66 | 0.969818 |
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Target: 5'- --cGACCUGUcccaugcuucGUCGUCGACGcCGUUGu -3' miRNA: 3'- gucUUGGGCG----------CAGCAGCUGCuGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 24699 | 0.67 | 0.957059 |
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Target: 5'- aGGAGCCcCGCGaggUGUUGACGuCGUUu -3' miRNA: 3'- gUCUUGG-GCGCa--GCAGCUGCuGCAGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 12792 | 0.66 | 0.979607 |
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Target: 5'- cCAGGccgGCCgGCGUuuucacuacgcCGUgGACGACGcCGa -3' miRNA: 3'- -GUCU---UGGgCGCA-----------GCAgCUGCUGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 7625 | 0.68 | 0.941037 |
|
Target: 5'- uCAGAgaccGCgCCGuCGUUGUUGACGAUGcCGc -3' miRNA: 3'- -GUCU----UG-GGC-GCAGCAGCUGCUGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 1959 | 0.68 | 0.94536 |
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Target: 5'- gCGGGGCCgGCGaCGggGACGGCGgCGg -3' miRNA: 3'- -GUCUUGGgCGCaGCagCUGCUGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 1853 | 0.66 | 0.977419 |
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Target: 5'- ----cCCCGCGUCGcugcUGACGgccguGCGUCGg -3' miRNA: 3'- gucuuGGGCGCAGCa---GCUGC-----UGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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