Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1618 | 3' | -55.6 | NC_001347.2 | + | 137713 | 0.67 | 0.96383 |
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Target: 5'- gUAGAGCCgGCGUCGccacauUCGAUGGUGUaCGc -3' miRNA: 3'- -GUCUUGGgCGCAGC------AGCUGCUGCA-GC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 109456 | 0.67 | 0.960545 |
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Target: 5'- aAGAcgcCCCGCGUCaccggCGGCGGCGcCa -3' miRNA: 3'- gUCUu--GGGCGCAGca---GCUGCUGCaGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 182214 | 0.66 | 0.969818 |
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Target: 5'- gCGGG--CUGCGUCGccuUCGGUGACGUCGg -3' miRNA: 3'- -GUCUugGGCGCAGC---AGCUGCUGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 25131 | 0.66 | 0.969818 |
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Target: 5'- --cGACCUGUcccaugcuucGUCGUCGACGcCGUUGu -3' miRNA: 3'- gucUUGGGCG----------CAGCAGCUGCuGCAGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 196572 | 0.66 | 0.969818 |
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Target: 5'- --cAACCCGCGUCG-CGGCuACGcCa -3' miRNA: 3'- gucUUGGGCGCAGCaGCUGcUGCaGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 39417 | 0.66 | 0.97253 |
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Target: 5'- cCAGuGCCCGC--UGUUGACGGCGcUCc -3' miRNA: 3'- -GUCuUGGGCGcaGCAGCUGCUGC-AGc -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 67427 | 0.66 | 0.974321 |
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Target: 5'- gGGAGCgaaagaagugacgaCgGCGaCGUCGACGACGgCGa -3' miRNA: 3'- gUCUUG--------------GgCGCaGCAGCUGCUGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 78356 | 0.66 | 0.97253 |
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Target: 5'- -cGGACCCgGCGUgGUgggaccCGGCGGCGcCGu -3' miRNA: 3'- guCUUGGG-CGCAgCA------GCUGCUGCaGC- -5' |
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| 1618 | 3' | -55.6 | NC_001347.2 | + | 95099 | 0.66 | 0.981635 |
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Target: 5'- aCAGGcGCCCGaggCGUCGcacAUGACGUCu -3' miRNA: 3'- -GUCU-UGGGCgcaGCAGC---UGCUGCAGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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