Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16180 | 5' | -54.4 | NC_004065.1 | + | 21851 | 0.71 | 0.846081 |
Target: 5'- aGUUGGUCGgaUGCCUCgUUGUACacaCCGCGg -3' miRNA: 3'- -CGACCAGC--GCGGAG-AAUAUG---GGUGCa -5' |
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16180 | 5' | -54.4 | NC_004065.1 | + | 195783 | 0.77 | 0.531705 |
Target: 5'- cGCaGGUCGCGUCUCUgg-GCgCCGCGg -3' miRNA: 3'- -CGaCCAGCGCGGAGAauaUG-GGUGCa -5' |
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16180 | 5' | -54.4 | NC_004065.1 | + | 94402 | 0.79 | 0.412332 |
Target: 5'- gGCUGGUgGCGCCg--UAgACCCGCGUg -3' miRNA: 3'- -CGACCAgCGCGGagaAUaUGGGUGCA- -5' |
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16180 | 5' | -54.4 | NC_004065.1 | + | 182721 | 1.1 | 0.005205 |
Target: 5'- cGCUGGUCGCGCCUCUUAUACCCACGUa -3' miRNA: 3'- -CGACCAGCGCGGAGAAUAUGGGUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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