Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16183 | 5' | -51 | NC_004065.1 | + | 221917 | 0.66 | 0.996918 |
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Target: 5'- cGGCUC---UGGGGCga-CUUGUUGGg -3' miRNA: 3'- aCCGAGguuACCCUGaaaGGACAACU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 1824 | 0.66 | 0.996918 |
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Target: 5'- cGGUUCCcGUGGuGACggugaUCgUGUUGGu -3' miRNA: 3'- aCCGAGGuUACC-CUGaa---AGgACAACU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 46183 | 0.67 | 0.995067 |
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Target: 5'- cGGC-CCAuaGGGACUUUCCa----- -3' miRNA: 3'- aCCGaGGUuaCCCUGAAAGGacaacu -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 221876 | 0.68 | 0.988694 |
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Target: 5'- gGGCUugggucgcgCCGGUGGGACUggUUUCUGgguagGAu -3' miRNA: 3'- aCCGA---------GGUUACCCUGA--AAGGACaa---CU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 114913 | 0.68 | 0.985533 |
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Target: 5'- gUGGCUCCGggggGUGGGGCggcacaUUUCUGg--- -3' miRNA: 3'- -ACCGAGGU----UACCCUGa-----AAGGACaacu -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 46424 | 0.69 | 0.981736 |
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Target: 5'- gGGacuuUCCAAUGGGuuUUUCCauUGUUGGc -3' miRNA: 3'- aCCg---AGGUUACCCugAAAGG--ACAACU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 183393 | 0.79 | 0.621978 |
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Target: 5'- uUGGCUCCAAUGGGuuUUUCCcaUUGAc -3' miRNA: 3'- -ACCGAGGUUACCCugAAAGGacAACU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 183300 | 1.1 | 0.011074 |
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Target: 5'- uUGGCUCCAAUGGGACUUUCCUGUUGAc -3' miRNA: 3'- -ACCGAGGUUACCCUGAAAGGACAACU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 183486 | 1.1 | 0.010766 |
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Target: 5'- uUGGCUCCAAUGGGACUUUCCUGUUGAu -3' miRNA: 3'- -ACCGAGGUUACCCUGAAAGGACAACU- -5' |
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| 16183 | 5' | -51 | NC_004065.1 | + | 183115 | 1.1 | 0.010766 |
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Target: 5'- uUGGCUCCAAUGGGACUUUCCUGUUGAu -3' miRNA: 3'- -ACCGAGGUUACCCUGAAAGGACAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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