Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
16207 | 3' | -59.1 | NC_004065.1 | + | 69606 | 0.7 | 0.605843 |
Target: 5'- --gGUGUaCCGCCGGGUGCAG-GUGg -3' miRNA: 3'- caaCGCGcGGUGGUCCACGUCgCACa -5' |
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16207 | 3' | -59.1 | NC_004065.1 | + | 83220 | 0.72 | 0.481146 |
Target: 5'- -gUGCGCGCCGCCGGGaGgAGCa--- -3' miRNA: 3'- caACGCGCGGUGGUCCaCgUCGcaca -5' |
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16207 | 3' | -59.1 | NC_004065.1 | + | 124535 | 0.73 | 0.42781 |
Target: 5'- --cGUGCGCCACCAGGUGgaaCAGCu--- -3' miRNA: 3'- caaCGCGCGGUGGUCCAC---GUCGcaca -5' |
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16207 | 3' | -59.1 | NC_004065.1 | + | 198639 | 1.06 | 0.003175 |
Target: 5'- gGUUGCGCGCCACCAGGUGCAGCGUGUc -3' miRNA: 3'- -CAACGCGCGGUGGUCCACGUCGCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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