Results 81 - 87 of 87 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16215 | 3' | -63.8 | NC_004065.1 | + | 30755 | 0.68 | 0.526445 |
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Target: 5'- -gGCUGCCCUCG-GaAUCCGAGGucggcggacgcggacGCCGa -3' miRNA: 3'- uaCGGCGGGAGCgC-UGGGCUCC---------------UGGC- -5' |
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| 16215 | 3' | -63.8 | NC_004065.1 | + | 29691 | 0.67 | 0.61583 |
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Target: 5'- uUGCUGagCCUCgGCGcCCCGAcGGGCUGg -3' miRNA: 3'- uACGGCg-GGAG-CGCuGGGCU-CCUGGC- -5' |
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| 16215 | 3' | -63.8 | NC_004065.1 | + | 29012 | 0.7 | 0.435694 |
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Target: 5'- -cGCCGCgCgcaCGCGGCCuCGAGucaGACCGg -3' miRNA: 3'- uaCGGCGgGa--GCGCUGG-GCUC---CUGGC- -5' |
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| 16215 | 3' | -63.8 | NC_004065.1 | + | 26672 | 0.67 | 0.61583 |
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Target: 5'- cGUGUCGCCC--GUGACCCuuuuugauGAGGACgGa -3' miRNA: 3'- -UACGGCGGGagCGCUGGG--------CUCCUGgC- -5' |
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| 16215 | 3' | -63.8 | NC_004065.1 | + | 18555 | 0.66 | 0.6912 |
|
Target: 5'- -cGCCGacgaCCUCuccgGCGACuccuCCGAGGAgCGg -3' miRNA: 3'- uaCGGCg---GGAG----CGCUG----GGCUCCUgGC- -5' |
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| 16215 | 3' | -63.8 | NC_004065.1 | + | 4866 | 0.69 | 0.50479 |
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Target: 5'- -cGCCGCCCUCGgcuCGgucACCCagGAGGAgCGu -3' miRNA: 3'- uaCGGCGGGAGC---GC---UGGG--CUCCUgGC- -5' |
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| 16215 | 3' | -63.8 | NC_004065.1 | + | 447 | 0.85 | 0.046074 |
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Target: 5'- -aGCCGCCCUCGCGGCguaacguCCGGGGACCc -3' miRNA: 3'- uaCGGCGGGAGCGCUG-------GGCUCCUGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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