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miRNA: 3'- -GAGCAGCaCGCUGCC-CCaGCa-GUUGC- -5'
miRNA: 3'- -GAGCAGCACG---CU--------------GCCCCA-GCAGUUGC- -5'
miRNA: 3'- -GAGCAGCa--CGCuGCCCCAGC-------------AGUUGC- -5'
miRNA: 3'- -GAGCAGCACGCUGCCCCAGCAGUUGC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 16219 | 3' | -56 | NC_004065.1 | + | 200779 | 0.75 | 0.614595 |
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Target: 5'- uCUCGUCGcGCGGCGGuGG-CGggCGACGg -3' miRNA: 3'- -GAGCAGCaCGCUGCC-CCaGCa-GUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 120327 | 0.76 | 0.515868 |
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Target: 5'- cCUCGUCGUGCuccGAgaaucucuccaccuCGGGGUaCGUCGGCGc -3' miRNA: 3'- -GAGCAGCACG---CU--------------GCCCCA-GCAGUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 102548 | 0.79 | 0.387223 |
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Target: 5'- cCUCGUCGgugGCGcCGGGGUCGggucccgcggccaUCAGCGu -3' miRNA: 3'- -GAGCAGCa--CGCuGCCCCAGC-------------AGUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 207702 | 1.08 | 0.0063 |
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Target: 5'- cCUCGUCGUGCGACGGGGUCGUCAACGu -3' miRNA: 3'- -GAGCAGCACGCUGCCCCAGCAGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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