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miRNA: 3'- gagCaGCACGCuGCCCCaGCAGU--UGC- -5'
miRNA: 3'- gAGCAGCac---GCUGCC-CCAgCaGUUGC- -5'
miRNA: 3'- gAGCaGCACGcu--GCCCCAGca-GUUGC- -5'
miRNA: 3'- gAGCaGCaCGCuGCC----CCAGCAGUUGc -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 16219 | 3' | -56 | NC_004065.1 | + | 41484 | 0.66 | 0.963827 |
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Target: 5'- --aG-CGUGCGcCGGGG-CGUCGggACGg -3' miRNA: 3'- gagCaGCACGCuGCCCCaGCAGU--UGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 62355 | 0.66 | 0.969815 |
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Target: 5'- gUCGUCGguuuuCGACGGcGGUgG-CGGCGg -3' miRNA: 3'- gAGCAGCac---GCUGCC-CCAgCaGUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 45092 | 0.66 | 0.966917 |
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Target: 5'- uUCGaCGUGCagcuCGGGGUCcucCAGCGg -3' miRNA: 3'- gAGCaGCACGcu--GCCCCAGca-GUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 143113 | 0.66 | 0.975059 |
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Target: 5'- uUCG-CG-GCGcCGGgaaaGGUCGUCGACa -3' miRNA: 3'- gAGCaGCaCGCuGCC----CCAGCAGUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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