miRNA: 3'- gaGCAGCACGC-----UGCCc---CA-GCAGUUGC- -5'
miRNA: 3'- gAGCAGCacGCUGCCC--------CAGCAGuUGC- -5'
miRNA: 3'- -GAGCAGCAcgcuGCC-CCAG-CAGUUGC- -5'
miRNA: 3'- gAGCaGCaCGCuGCC----CCAGCAGUUGc -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16219 | 3' | -56 | NC_004065.1 | + | 187909 | 0.66 | 0.972528 |
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Target: 5'- -cCGUCGUGCGccaaaACGGucacGUaCGUCGGCGc -3' miRNA: 3'- gaGCAGCACGC-----UGCCc---CA-GCAGUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 19770 | 0.66 | 0.975059 |
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Target: 5'- gUCGUCGauCGAUGGGcaguucgaGUCGUCcGCGg -3' miRNA: 3'- gAGCAGCacGCUGCCC--------CAGCAGuUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 77230 | 0.66 | 0.975059 |
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Target: 5'- cCUCGUCGU----CGGaGGUCaGUCGACGu -3' miRNA: 3'- -GAGCAGCAcgcuGCC-CCAG-CAGUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 143113 | 0.66 | 0.975059 |
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Target: 5'- uUCG-CG-GCGcCGGgaaaGGUCGUCGACa -3' miRNA: 3'- gAGCaGCaCGCuGCC----CCAGCAGUUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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