miRNA: 3'- -GAGCaGCaCGCUGCCCCAGcAGUUgc -5'
miRNA: 3'- gAGCAGCAcGCU-GC-CCCAGCaGUUGC- -5'
miRNA: 3'- -GAgCAGCa-C-GCUGCCCCaGCAGU-UGC- -5'
miRNA: 3'- -GAGCAGCa--CGCuGCCCCAGC-------------AGUUGC- -5'
Results 61 - 64 of 64 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16219 | 3' | -56 | NC_004065.1 | + | 202277 | 0.68 | 0.910547 |
|
Target: 5'- uCUCGaUGcGCGGCGGGGUCaUCAGa- -3' miRNA: 3'- -GAGCaGCaCGCUGCCCCAGcAGUUgc -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 186738 | 0.68 | 0.921577 |
|
Target: 5'- gUCGUCGUcUGGuCGcGGGUCGaCGACGa -3' miRNA: 3'- gAGCAGCAcGCU-GC-CCCAGCaGUUGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 82983 | 0.68 | 0.926767 |
|
Target: 5'- cCUgGUCGcaGuCGACGGGG-CGUCGcACGa -3' miRNA: 3'- -GAgCAGCa-C-GCUGCCCCaGCAGU-UGC- -5' |
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| 16219 | 3' | -56 | NC_004065.1 | + | 102548 | 0.79 | 0.387223 |
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Target: 5'- cCUCGUCGgugGCGcCGGGGUCGggucccgcggccaUCAGCGu -3' miRNA: 3'- -GAGCAGCa--CGCuGCCCCAGC-------------AGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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