Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1622 | 3' | -51.2 | NC_001347.2 | + | 191047 | 0.67 | 0.994603 |
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Target: 5'- aUCGUGCCGCG-GAAC-UGCg------ -3' miRNA: 3'- -AGCGCGGCGCuCUUGcACGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 199889 | 0.67 | 0.996003 |
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Target: 5'- -gGCGCUGCGcGcccGCGUGCUcg-UGAa -3' miRNA: 3'- agCGCGGCGCuCu--UGCACGAaaaACU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 107809 | 0.66 | 0.996582 |
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Target: 5'- aUCGCGCUG-GAGGACcUGCUg----- -3' miRNA: 3'- -AGCGCGGCgCUCUUGcACGAaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 2485 | 0.66 | 0.99709 |
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Target: 5'- gCGCGCCGCGcuguGGGCGcGCg------ -3' miRNA: 3'- aGCGCGGCGCu---CUUGCaCGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 12720 | 0.66 | 0.99709 |
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Target: 5'- gUCuCGCCGCGAGAucugGCGguugGCUgacagcgUGGa -3' miRNA: 3'- -AGcGCGGCGCUCU----UGCa---CGAaaa----ACU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 172379 | 0.66 | 0.997534 |
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Target: 5'- -gGCgGCCGUGAaccuGAGCGUGUUUgugGAc -3' miRNA: 3'- agCG-CGGCGCU----CUUGCACGAAaaaCU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 82822 | 0.66 | 0.997921 |
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Target: 5'- cCGCGUCGUGAGcGCGgcgGCUc----- -3' miRNA: 3'- aGCGCGGCGCUCuUGCa--CGAaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 174963 | 0.66 | 0.998542 |
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Target: 5'- aCGUGCuCGCu-GAACGUGCUg----- -3' miRNA: 3'- aGCGCG-GCGcuCUUGCACGAaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 156227 | 0.66 | 0.998542 |
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Target: 5'- gCGCGCCacguagGUGAGuucAAUGUGCUUaaggUGAa -3' miRNA: 3'- aGCGCGG------CGCUC---UUGCACGAAaa--ACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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