Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1622 | 3' | -51.2 | NC_001347.2 | + | 38862 | 0.7 | 0.973497 |
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Target: 5'- gCGCGCCGCuAGGaucaGCGUGUUgUUUGc -3' miRNA: 3'- aGCGCGGCGcUCU----UGCACGAaAAACu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 182859 | 0.7 | 0.967712 |
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Target: 5'- gCGCGCCGUGGGAuCG-GCUUc---- -3' miRNA: 3'- aGCGCGGCGCUCUuGCaCGAAaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 167228 | 0.7 | 0.961084 |
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Target: 5'- cCGCGCCGCGAG-ACGgacGCa------ -3' miRNA: 3'- aGCGCGGCGCUCuUGCa--CGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 5567 | 0.72 | 0.93575 |
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Target: 5'- gUUGCGCgGUGGGGGCGUGCc------ -3' miRNA: 3'- -AGCGCGgCGCUCUUGCACGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 171387 | 0.72 | 0.914095 |
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Target: 5'- uUCGCGCUGCGAGcaauCGUGCcccaaggUGGu -3' miRNA: 3'- -AGCGCGGCGCUCuu--GCACGaaaa---ACU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 165545 | 0.73 | 0.901827 |
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Target: 5'- -aGgGCCGCGAGAgcGCGUGCa------ -3' miRNA: 3'- agCgCGGCGCUCU--UGCACGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 140333 | 0.73 | 0.901827 |
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Target: 5'- cUGCGCCGCGGGGcUGUGCgaggccugUGGg -3' miRNA: 3'- aGCGCGGCGCUCUuGCACGaaaa----ACU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 203350 | 0.74 | 0.859501 |
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Target: 5'- aCGCGCCGCGAGcuCGUcggagGCUUUUc-- -3' miRNA: 3'- aGCGCGGCGCUCuuGCA-----CGAAAAacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 142089 | 1.1 | 0.011698 |
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Target: 5'- cUCGCGCCGCGAGAACGUGCUUUUUGAc -3' miRNA: 3'- -AGCGCGGCGCUCUUGCACGAAAAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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