Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1622 | 3' | -51.2 | NC_001347.2 | + | 2485 | 0.66 | 0.99709 |
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Target: 5'- gCGCGCCGCGcuguGGGCGcGCg------ -3' miRNA: 3'- aGCGCGGCGCu---CUUGCaCGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 107809 | 0.66 | 0.996582 |
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Target: 5'- aUCGCGCUG-GAGGACcUGCUg----- -3' miRNA: 3'- -AGCGCGGCgCUCUUGcACGAaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 191047 | 0.67 | 0.994603 |
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Target: 5'- aUCGUGCCGCG-GAAC-UGCg------ -3' miRNA: 3'- -AGCGCGGCGCuCUUGcACGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 199889 | 0.67 | 0.996003 |
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Target: 5'- -gGCGCUGCGcGcccGCGUGCUcg-UGAa -3' miRNA: 3'- agCGCGGCGCuCu--UGCACGAaaaACU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 198582 | 0.68 | 0.991784 |
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Target: 5'- gCGUGCuCGCGuAGAgcGCGUGUcguagUUUUGAc -3' miRNA: 3'- aGCGCG-GCGC-UCU--UGCACGa----AAAACU- -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 53743 | 0.67 | 0.99283 |
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Target: 5'- gCGUGCCGCGGGuGCGcGCa------ -3' miRNA: 3'- aGCGCGGCGCUCuUGCaCGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 153868 | 0.67 | 0.994603 |
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Target: 5'- cCGCGCCgaGCGGcGACGUGCa------ -3' miRNA: 3'- aGCGCGG--CGCUcUUGCACGaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 88367 | 0.68 | 0.989333 |
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Target: 5'- gCGCuGCCGCGAGAcgACGUGg------- -3' miRNA: 3'- aGCG-CGGCGCUCU--UGCACgaaaaacu -5' |
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| 1622 | 3' | -51.2 | NC_001347.2 | + | 203350 | 0.74 | 0.859501 |
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Target: 5'- aCGCGCCGCGAGcuCGUcggagGCUUUUc-- -3' miRNA: 3'- aGCGCGGCGCUCuuGCA-----CGAAAAacu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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