Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16222 | 3' | -64.1 | NC_004065.1 | + | 51520 | 0.66 | 0.64638 |
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Target: 5'- aGGCCAUGaCGAcGCCGucGCGUCCGaUGa -3' miRNA: 3'- gCCGGUAC-GCU-CGGUc-CGCGGGCcAC- -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 61719 | 0.66 | 0.665299 |
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Target: 5'- gCGGCgGaUGCGAGCCAuGCGCUccuuggCGGg- -3' miRNA: 3'- -GCCGgU-ACGCUCGGUcCGCGG------GCCac -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 29493 | 0.66 | 0.64638 |
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Target: 5'- uGGUCAgGCGuuCCAGGUaCUCGGUGa -3' miRNA: 3'- gCCGGUaCGCucGGUCCGcGGGCCAC- -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 110834 | 0.66 | 0.636903 |
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Target: 5'- aGGCaccaGUGCgGGGCgCGGGcCGCCCGcGUc -3' miRNA: 3'- gCCGg---UACG-CUCG-GUCC-GCGGGC-CAc -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 27462 | 0.66 | 0.636903 |
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Target: 5'- -cGCgCAUcacgcGCGAGCuCGGGUuccuGCCCGGUGu -3' miRNA: 3'- gcCG-GUA-----CGCUCG-GUCCG----CGGGCCAC- -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 77984 | 0.66 | 0.617945 |
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Target: 5'- uGGCCGcgGCGAGCC--GC-CCCGGg- -3' miRNA: 3'- gCCGGUa-CGCUCGGucCGcGGGCCac -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 151160 | 0.67 | 0.608477 |
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Target: 5'- uCGGCgAccGCGAGCU-GGCGCgCCGGc- -3' miRNA: 3'- -GCCGgUa-CGCUCGGuCCGCG-GGCCac -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 109851 | 0.67 | 0.599024 |
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Target: 5'- gGGCCAUcGCGcagguGCCGGGCGgugccaCCGGc- -3' miRNA: 3'- gCCGGUA-CGCu----CGGUCCGCg-----GGCCac -5' |
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| 16222 | 3' | -64.1 | NC_004065.1 | + | 211479 | 1.07 | 0.001095 |
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Target: 5'- uCGGCCAUGCGAGCCAGGCGCCCGGUGa -3' miRNA: 3'- -GCCGGUACGCUCGGUCCGCGGGCCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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