Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16223 | 5' | -57 | NC_004065.1 | + | 36176 | 0.66 | 0.91677 |
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Target: 5'- -gUCAGCCGCUCG-CGGGGggGcgCg- -3' miRNA: 3'- caGGUCGGCGAGCuGCUCCa-CuaGag -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 211142 | 0.66 | 0.918984 |
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Target: 5'- gGUCUGGCCGUUCGGgGGGGagcgggcgugccggGAUgUCg -3' miRNA: 3'- -CAGGUCGGCGAGCUgCUCCa-------------CUAgAG- -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 187172 | 0.66 | 0.922239 |
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Target: 5'- cGUCCcuCgCGCUCucCGAGG-GGUCUCg -3' miRNA: 3'- -CAGGucG-GCGAGcuGCUCCaCUAGAG- -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 134259 | 0.66 | 0.922239 |
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Target: 5'- -aCCgAGCCGCacgUCGACGAGaucaauGUGGUCg- -3' miRNA: 3'- caGG-UCGGCG---AGCUGCUC------CACUAGag -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 67682 | 0.66 | 0.927484 |
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Target: 5'- -cCCGGUCcgaucgGCUCGAUGAGGgGGUCg- -3' miRNA: 3'- caGGUCGG------CGAGCUGCUCCaCUAGag -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 164220 | 0.66 | 0.927484 |
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Target: 5'- -aCgAGCCGCaUGACGGcGGUGAagUCg -3' miRNA: 3'- caGgUCGGCGaGCUGCU-CCACUagAG- -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 127411 | 0.66 | 0.932504 |
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Target: 5'- cUCCcaGGCCGUcaccgUCGACGAGGagcacAUCUCc -3' miRNA: 3'- cAGG--UCGGCG-----AGCUGCUCCac---UAGAG- -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 98450 | 0.66 | 0.932504 |
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Target: 5'- -aCCAGUCGgaCGACcuGGUGGUCg- -3' miRNA: 3'- caGGUCGGCgaGCUGcuCCACUAGag -5' |
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| 16223 | 5' | -57 | NC_004065.1 | + | 165659 | 0.66 | 0.937299 |
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Target: 5'- cUCCGGCCGCgcccUGAgGAcGGUcGGUUUCu -3' miRNA: 3'- cAGGUCGGCGa---GCUgCU-CCA-CUAGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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