Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16225 | 3' | -43.1 | NC_004065.1 | + | 83910 | 0.66 | 1 |
Target: 5'- gAUGUCGCGUGAgacgaagCcgCGGCGGAg- -3' miRNA: 3'- gUAUAGUGUACUaaa----Ga-GCUGUCUag -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 182040 | 0.69 | 1 |
Target: 5'- gCAUGUCGCG-GAUgaccagUUUCGgggaGCAGAUCg -3' miRNA: 3'- -GUAUAGUGUaCUAa-----AGAGC----UGUCUAG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 55085 | 0.66 | 1 |
Target: 5'- ----gUACcgGAUUUUUCGguACAGAUCu -3' miRNA: 3'- guauaGUGuaCUAAAGAGC--UGUCUAG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 79904 | 0.66 | 1 |
Target: 5'- aCGUGUCGCG-GAUc-CUCGGC-GAUCu -3' miRNA: 3'- -GUAUAGUGUaCUAaaGAGCUGuCUAG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 89597 | 0.66 | 1 |
Target: 5'- --aGUC-CAUGAUgcgcucgaaCUCGACGGAUUu -3' miRNA: 3'- guaUAGuGUACUAaa-------GAGCUGUCUAG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 88700 | 0.67 | 1 |
Target: 5'- gCGUcgUACAUGAcgUUCUCG-CGGcgCg -3' miRNA: 3'- -GUAuaGUGUACUa-AAGAGCuGUCuaG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 6885 | 0.68 | 1 |
Target: 5'- --cGUCACGUguaaacugacGAUUUgUCGACAGAc- -3' miRNA: 3'- guaUAGUGUA----------CUAAAgAGCUGUCUag -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 12363 | 0.67 | 1 |
Target: 5'- --gAUCAUcgGAUacCUCGACAGGa- -3' miRNA: 3'- guaUAGUGuaCUAaaGAGCUGUCUag -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 15999 | 0.69 | 0.999999 |
Target: 5'- ----aCACAUGAUgUCcUGACAGAUUa -3' miRNA: 3'- guauaGUGUACUAaAGaGCUGUCUAG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 173653 | 0.73 | 0.999716 |
Target: 5'- uGUGUCGgAUGGUcucaUCUCGACGGAa- -3' miRNA: 3'- gUAUAGUgUACUAa---AGAGCUGUCUag -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 158522 | 0.74 | 0.999544 |
Target: 5'- --cGUCGCAUGcugacagUCUgGACAGGUCg -3' miRNA: 3'- guaUAGUGUACuaa----AGAgCUGUCUAG- -5' |
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16225 | 3' | -43.1 | NC_004065.1 | + | 213988 | 1.12 | 0.051154 |
Target: 5'- uCAUAUCACAUGAUUUCUCGACAGAUCg -3' miRNA: 3'- -GUAUAGUGUACUAAAGAGCUGUCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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