Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16233 | 5' | -48.9 | NC_004065.1 | + | 197642 | 0.71 | 0.981244 |
Target: 5'- cGGCgGCaCCGgGCUCAGCU--UGUACa -3' miRNA: 3'- -CUGgCGaGGUgUGAGUCGAaaAUAUG- -5' |
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16233 | 5' | -48.9 | NC_004065.1 | + | 204018 | 0.66 | 0.999721 |
Target: 5'- cGCCGaggCCACGgUCAGCgacgugcUGUACg -3' miRNA: 3'- cUGGCga-GGUGUgAGUCGaaa----AUAUG- -5' |
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16233 | 5' | -48.9 | NC_004065.1 | + | 225631 | 0.66 | 0.999721 |
Target: 5'- cACCGCUCCgaacaGCACUcCGGCUc------ -3' miRNA: 3'- cUGGCGAGG-----UGUGA-GUCGAaaauaug -5' |
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16233 | 5' | -48.9 | NC_004065.1 | + | 225671 | 0.67 | 0.998973 |
Target: 5'- --gCGCUCCACGCgCAGCcgUUUGg-- -3' miRNA: 3'- cugGCGAGGUGUGaGUCGa-AAAUaug -5' |
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16233 | 5' | -48.9 | NC_004065.1 | + | 228874 | 1.12 | 0.012282 |
Target: 5'- cGACCGCUCCACACUCAGCUUUUAUACa -3' miRNA: 3'- -CUGGCGAGGUGUGAGUCGAAAAUAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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