Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16249 | 3' | -59.4 | NC_004084.1 | + | 57140 | 0.66 | 0.525862 |
Target: 5'- cGAGCCGGGCA---UCCccGGGCGaUCa- -3' miRNA: 3'- -CUUGGCCCGUugcAGGa-CCCGC-AGga -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 36247 | 0.66 | 0.495406 |
Target: 5'- -uACCGGGuCGACGUCCUcGaGuGCG-CCg -3' miRNA: 3'- cuUGGCCC-GUUGCAGGA-C-C-CGCaGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 51946 | 0.66 | 0.482458 |
Target: 5'- cAGCCGGGCGAgGUCCaggacaucccgcUccacggcgaccaguGGGCGUUCg -3' miRNA: 3'- cUUGGCCCGUUgCAGG------------A--------------CCCGCAGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 49140 | 0.67 | 0.475553 |
Target: 5'- cGGACCaGGCGACGUUCgauGCGUUCa -3' miRNA: 3'- -CUUGGcCCGUUGCAGGaccCGCAGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 10680 | 0.67 | 0.475553 |
Target: 5'- -cGCCGGGCAGCGaCCguucGGGUagCCg -3' miRNA: 3'- cuUGGCCCGUUGCaGGa---CCCGcaGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 29398 | 0.68 | 0.374339 |
Target: 5'- --gUCGGGCGGCcacUCCUGGGgCGUCg- -3' miRNA: 3'- cuuGGCCCGUUGc--AGGACCC-GCAGga -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 34701 | 0.69 | 0.341325 |
Target: 5'- -cGCUGGGguGuCGUCCUuuccGGGCGUCg- -3' miRNA: 3'- cuUGGCCCguU-GCAGGA----CCCGCAGga -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 15227 | 0.71 | 0.268242 |
Target: 5'- -cACCGGGCAuCGUCCaccuccucGGGCGacUCCg -3' miRNA: 3'- cuUGGCCCGUuGCAGGa-------CCCGC--AGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 56834 | 0.73 | 0.19782 |
Target: 5'- cAGCCGGGCGAcguCGUCCUGGaccCGUUCg -3' miRNA: 3'- cUUGGCCCGUU---GCAGGACCc--GCAGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 5361 | 0.77 | 0.092878 |
Target: 5'- aGGACUGGGacgaucuCGACGUcgaaCCUGGGCGUCCa -3' miRNA: 3'- -CUUGGCCC-------GUUGCA----GGACCCGCAGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 53274 | 0.84 | 0.032837 |
Target: 5'- cGGGCCGGGCGACGUCCU-GGCGaCCa -3' miRNA: 3'- -CUUGGCCCGUUGCAGGAcCCGCaGGa -5' |
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16249 | 3' | -59.4 | NC_004084.1 | + | 57957 | 1.07 | 0.000625 |
Target: 5'- cGAACCGGGCAACGUCCUGGGCGUCCUc -3' miRNA: 3'- -CUUGGCCCGUUGCAGGACCCGCAGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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