Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16252 | 3' | -53 | NC_004084.1 | + | 35470 | 0.66 | 0.860343 |
Target: 5'- cCAGGAGCaGGUcGAggCGGCgAUCgcCGAg -3' miRNA: 3'- -GUCUUCG-CCA-CUa-GCCGgUAGaaGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 20562 | 0.66 | 0.851893 |
Target: 5'- gAGGAGaCGGgccaguucgaAUCGGCCGUCgaCGAa -3' miRNA: 3'- gUCUUC-GCCac--------UAGCCGGUAGaaGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 20904 | 0.67 | 0.776592 |
Target: 5'- -cGGAGCGGUGAcCGGUuccuggaugcuCGUCUguUCGGg -3' miRNA: 3'- guCUUCGCCACUaGCCG-----------GUAGA--AGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 27459 | 0.68 | 0.756012 |
Target: 5'- -cGGAGCGGgagacGGUCGG-CGUCcUCGAg -3' miRNA: 3'- guCUUCGCCa----CUAGCCgGUAGaAGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 56788 | 0.68 | 0.745521 |
Target: 5'- gCAGAAGCc--GGUCGGCC-UCcUCGAg -3' miRNA: 3'- -GUCUUCGccaCUAGCCGGuAGaAGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 18832 | 0.68 | 0.724199 |
Target: 5'- aGGAGGaCGGUGGcgCGGCUuUCgUCGAc -3' miRNA: 3'- gUCUUC-GCCACUa-GCCGGuAGaAGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 17286 | 0.69 | 0.69154 |
Target: 5'- uGGAGGCGGUGG-CGGCgGUggCggCGAg -3' miRNA: 3'- gUCUUCGCCACUaGCCGgUA--GaaGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 25083 | 0.69 | 0.658353 |
Target: 5'- -cGAcAGUGGUGGUaCGGUCGUCaUUCGGc -3' miRNA: 3'- guCU-UCGCCACUA-GCCGGUAG-AAGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 33218 | 0.69 | 0.658353 |
Target: 5'- aCAGAGGCGGccGAgcgGGCCGUCcccuacgagUUCGAu -3' miRNA: 3'- -GUCUUCGCCa-CUag-CCGGUAG---------AAGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 7346 | 0.71 | 0.547633 |
Target: 5'- cCAG-AGUGG-GGUCaGGCCAUCUaCGAg -3' miRNA: 3'- -GUCuUCGCCaCUAG-CCGGUAGAaGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 35873 | 0.76 | 0.326437 |
Target: 5'- cCGGAc-CGcGUGAUCGGUCGUCUUCGGc -3' miRNA: 3'- -GUCUucGC-CACUAGCCGGUAGAAGCU- -5' |
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16252 | 3' | -53 | NC_004084.1 | + | 56795 | 1.09 | 0.001908 |
Target: 5'- cCAGAAGCGGUGAUCGGCCAUCUUCGAc -3' miRNA: 3'- -GUCUUCGCCACUAGCCGGUAGAAGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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