Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16253 | 5' | -55.2 | NC_004084.1 | + | 56731 | 1.07 | 0.001766 |
Target: 5'- cUCGGACGUCCUUCGCGUCGAUCAGGUc -3' miRNA: 3'- -AGCCUGCAGGAAGCGCAGCUAGUCCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 49211 | 0.73 | 0.371995 |
Target: 5'- cCGaACG-CCUUCGCGUCGAaCGGGa -3' miRNA: 3'- aGCcUGCaGGAAGCGCAGCUaGUCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 26252 | 0.7 | 0.515185 |
Target: 5'- -aGGGCGUCCUcgGCGUUGAUCAu-- -3' miRNA: 3'- agCCUGCAGGAagCGCAGCUAGUcca -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 35898 | 0.7 | 0.531848 |
Target: 5'- gUCGGACGUCCcgcuggaguggcCGCGUCGAgu-GGa -3' miRNA: 3'- -AGCCUGCAGGaa----------GCGCAGCUaguCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 22910 | 0.7 | 0.546586 |
Target: 5'- -aGGAUGUCCcacUCGCGaccgUUGGUCGGGa -3' miRNA: 3'- agCCUGCAGGa--AGCGC----AGCUAGUCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 19079 | 0.69 | 0.61094 |
Target: 5'- gCGGcCGUCUacgUCGaCGUCGAUCAGc- -3' miRNA: 3'- aGCCuGCAGGa--AGC-GCAGCUAGUCca -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 29099 | 0.68 | 0.632623 |
Target: 5'- -gGGACGUCCUcCGCGcCaucCAGGUa -3' miRNA: 3'- agCCUGCAGGAaGCGCaGcuaGUCCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 34452 | 0.68 | 0.654304 |
Target: 5'- uUCGGACGUaCUUacCGCGUCcAUCGcGGUg -3' miRNA: 3'- -AGCCUGCAgGAA--GCGCAGcUAGU-CCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 17916 | 0.67 | 0.675906 |
Target: 5'- gCGGGCugGUCCcaccUGCagGUCGAUCAGGUc -3' miRNA: 3'- aGCCUG--CAGGaa--GCG--CAGCUAGUCCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 18101 | 0.67 | 0.686651 |
Target: 5'- cUCGGACGUCaggUCGa---GGUCGGGg -3' miRNA: 3'- -AGCCUGCAGga-AGCgcagCUAGUCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 48026 | 0.67 | 0.686651 |
Target: 5'- gCGGA-GUUCUUCGCGcUCGAUCGc-- -3' miRNA: 3'- aGCCUgCAGGAAGCGC-AGCUAGUcca -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 7727 | 0.67 | 0.697346 |
Target: 5'- -aGGACGUCCUUCGCcUCGcu--GGc -3' miRNA: 3'- agCCUGCAGGAAGCGcAGCuaguCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 26652 | 0.67 | 0.729018 |
Target: 5'- cUCGGACGUaCUUacCGCGUCcAUCGcGGUc -3' miRNA: 3'- -AGCCUGCAgGAA--GCGCAGcUAGU-CCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 29701 | 0.66 | 0.738368 |
Target: 5'- gCGGACGcugUCCUggcugagUCGCGUCG-UCuGGc -3' miRNA: 3'- aGCCUGC---AGGA-------AGCGCAGCuAGuCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 9244 | 0.66 | 0.739402 |
Target: 5'- -aGGGCGUCCa--GCGUC--UCGGGg -3' miRNA: 3'- agCCUGCAGGaagCGCAGcuAGUCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 15679 | 0.66 | 0.739402 |
Target: 5'- cCGGGCcgauGUCCUUCGCcugcucgCGAUC-GGUg -3' miRNA: 3'- aGCCUG----CAGGAAGCGca-----GCUAGuCCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 53582 | 0.66 | 0.739402 |
Target: 5'- cCGGAUGUgC-UCGCGaCGAUCGGcGa -3' miRNA: 3'- aGCCUGCAgGaAGCGCaGCUAGUC-Ca -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 27473 | 0.66 | 0.749681 |
Target: 5'- gUCGG-CGUCCUcgagcUCGaCGUCGAcgAGGc -3' miRNA: 3'- -AGCCuGCAGGA-----AGC-GCAGCUagUCCa -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 9791 | 0.66 | 0.76988 |
Target: 5'- gUGGcuCGUCCUggccguaguucUCGCagucGUUGAUCAGGUu -3' miRNA: 3'- aGCCu-GCAGGA-----------AGCG----CAGCUAGUCCA- -5' |
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16253 | 5' | -55.2 | NC_004084.1 | + | 40482 | 0.66 | 0.779778 |
Target: 5'- uUCGGACGUCCcg-GUGUCaucuucuUCAGGc -3' miRNA: 3'- -AGCCUGCAGGaagCGCAGcu-----AGUCCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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