Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16254 | 3' | -61.8 | NC_004084.1 | + | 10968 | 0.66 | 0.412637 |
Target: 5'- uACGAccucgacccuUCUGgaCUGGAGGAGaUGcGGCUGACg -3' miRNA: 3'- -UGCU----------AGGC--GGCCUCCUC-AC-CCGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 37536 | 0.66 | 0.411747 |
Target: 5'- cCGA-CUGCCGGGgcugacugaccguGGAGUGGaCCGAUc -3' miRNA: 3'- uGCUaGGCGGCCU-------------CCUCACCcGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 18779 | 0.66 | 0.407316 |
Target: 5'- uGCGGUCgaGCCGGuuggagggaacauccAGGGcGUGuGGUCGGCg -3' miRNA: 3'- -UGCUAGg-CGGCC---------------UCCU-CAC-CCGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 2249 | 0.66 | 0.395072 |
Target: 5'- gGCGGagaagggccUCCgaaaGCUGGAGGAGaugGGGCUGGu -3' miRNA: 3'- -UGCU---------AGG----CGGCCUCCUCa--CCCGGCUg -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 11532 | 0.66 | 0.395072 |
Target: 5'- uGCag-CCGCCGG-GGcGUGGcggcaGCCGACg -3' miRNA: 3'- -UGcuaGGCGGCCuCCuCACC-----CGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 30113 | 0.66 | 0.377998 |
Target: 5'- gACG--UCGCUGG-GGAGcGGGCCGGg -3' miRNA: 3'- -UGCuaGGCGGCCuCCUCaCCCGGCUg -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 56973 | 0.66 | 0.377998 |
Target: 5'- aGCGGUCCGagCGaAGGAG-GcGGCCGAg -3' miRNA: 3'- -UGCUAGGCg-GCcUCCUCaC-CCGGCUg -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 6438 | 0.67 | 0.337534 |
Target: 5'- gUGGUgCGCCcGAGGAG-GGuGCUGGCg -3' miRNA: 3'- uGCUAgGCGGcCUCCUCaCC-CGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 5695 | 0.68 | 0.325269 |
Target: 5'- -aGGUCCGCUGGGuuGAGUucgaggacgucgcucGGGCCGAg -3' miRNA: 3'- ugCUAGGCGGCCUc-CUCA---------------CCCGGCUg -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 17325 | 0.68 | 0.300316 |
Target: 5'- uACGAUCgaggagaucaaCGCCGGAGGcGaGGGCuCGAg -3' miRNA: 3'- -UGCUAG-----------GCGGCCUCCuCaCCCG-GCUg -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 3643 | 0.68 | 0.300316 |
Target: 5'- gGCGAUCaguagCGCUGGcGGGGGuUGGGCUuGGCg -3' miRNA: 3'- -UGCUAG-----GCGGCC-UCCUC-ACCCGG-CUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 41803 | 0.69 | 0.245073 |
Target: 5'- cACGAUcgaccucgaggagcCCGCCGGguccguaGGGGGUGacgaGGCCGAUg -3' miRNA: 3'- -UGCUA--------------GGCGGCC-------UCCUCAC----CCGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 9689 | 0.72 | 0.164961 |
Target: 5'- gUGGcCCGCCGGAGaccgcgguGAGUuGGCCGACg -3' miRNA: 3'- uGCUaGGCGGCCUC--------CUCAcCCGGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 19000 | 0.72 | 0.160723 |
Target: 5'- -aGGUcCCGCUGGcGGGGUGGGaucCCGGCg -3' miRNA: 3'- ugCUA-GGCGGCCuCCUCACCC---GGCUG- -5' |
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16254 | 3' | -61.8 | NC_004084.1 | + | 55905 | 1.08 | 0.000314 |
Target: 5'- gACGAUCCGCCGGAGGAGUGGGCCGACu -3' miRNA: 3'- -UGCUAGGCGGCCUCCUCACCCGGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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