Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16255 | 3' | -51.2 | NC_004084.1 | + | 6335 | 0.66 | 0.936363 |
Target: 5'- aGAUCGUCGCgaGCGUUCUccauCCGcuGCu -3' miRNA: 3'- cUUAGCAGCG--CGCAAGGaaauGGC--CG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 48676 | 0.66 | 0.930887 |
Target: 5'- cGAagGUCGCGaCGUgggagaUCCUcgaagccGCCGGCa -3' miRNA: 3'- cUUagCAGCGC-GCA------AGGAaa-----UGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 57639 | 0.66 | 0.930887 |
Target: 5'- ----gGUCGCGCGUcgCCUcgacgagccgAUCGGCc -3' miRNA: 3'- cuuagCAGCGCGCAa-GGAaa--------UGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 4367 | 0.67 | 0.892053 |
Target: 5'- --cUCGUCGCGCG-UCCa--ACCGuaGCc -3' miRNA: 3'- cuuAGCAGCGCGCaAGGaaaUGGC--CG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 2348 | 0.67 | 0.889847 |
Target: 5'- aGAGUCGUCGUuccGCGUcgUCCUcgaagucguagaacUUgACCGGg -3' miRNA: 3'- -CUUAGCAGCG---CGCA--AGGA--------------AA-UGGCCg -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 19988 | 0.67 | 0.883846 |
Target: 5'- ---aCGUCGCGCGUUCgcucgaguucgcgCUUuaUGCCgaGGCc -3' miRNA: 3'- cuuaGCAGCGCGCAAG-------------GAA--AUGG--CCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 481 | 0.67 | 0.868913 |
Target: 5'- --uUCGUCGCcuCGaUCCU--GCUGGCg -3' miRNA: 3'- cuuAGCAGCGc-GCaAGGAaaUGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 8326 | 0.68 | 0.852205 |
Target: 5'- ---gCGUCgGCGCGgaugaUCCa--GCCGGCg -3' miRNA: 3'- cuuaGCAG-CGCGCa----AGGaaaUGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 37941 | 0.68 | 0.839937 |
Target: 5'- gGGAUCGUCGCGUuugaagacgaCUUcGCCGGUc -3' miRNA: 3'- -CUUAGCAGCGCGcaag------GAAaUGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 49486 | 0.68 | 0.832727 |
Target: 5'- gGAGUCGUCG-GCGUcaUCCggaucgucuUCGGCu -3' miRNA: 3'- -CUUAGCAGCgCGCA--AGGaaau-----GGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 16647 | 0.69 | 0.806424 |
Target: 5'- -cGUCGUCGaCGuCGUccgggaggUCCUcgucGCCGGCg -3' miRNA: 3'- cuUAGCAGC-GC-GCA--------AGGAaa--UGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 48329 | 0.69 | 0.786716 |
Target: 5'- uGAUCGUCGCGCcc-CCUUcguccUCGGCg -3' miRNA: 3'- cUUAGCAGCGCGcaaGGAAau---GGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 51351 | 0.7 | 0.766343 |
Target: 5'- --uUCGUCGacggguacaCGCuGUUCCUcUGCUGGCc -3' miRNA: 3'- cuuAGCAGC---------GCG-CAAGGAaAUGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 3919 | 0.72 | 0.624276 |
Target: 5'- uAGUCGUCGaagGCG-UCCUcgaACCGGCu -3' miRNA: 3'- cUUAGCAGCg--CGCaAGGAaa-UGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 46590 | 0.74 | 0.524977 |
Target: 5'- cGAUCGUCGUgcugaagcucGCGaUCCUcUACCGGUu -3' miRNA: 3'- cUUAGCAGCG----------CGCaAGGAaAUGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 3087 | 0.74 | 0.493103 |
Target: 5'- -cGUCGaCGUGCGUUCCg--GCgGGCu -3' miRNA: 3'- cuUAGCaGCGCGCAAGGaaaUGgCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 38507 | 0.77 | 0.363602 |
Target: 5'- cGAUCGUCGCGagcaucguucgaaGUUCCUguucuacACCGGCg -3' miRNA: 3'- cUUAGCAGCGCg------------CAAGGAaa-----UGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 29972 | 0.82 | 0.182221 |
Target: 5'- --cUCGUCGCGCGUgUCCUUguagaACCGGUu -3' miRNA: 3'- cuuAGCAGCGCGCA-AGGAAa----UGGCCG- -5' |
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16255 | 3' | -51.2 | NC_004084.1 | + | 55736 | 1.12 | 0.00197 |
Target: 5'- gGAAUCGUCGCGCGUUCCUUUACCGGCu -3' miRNA: 3'- -CUUAGCAGCGCGCAAGGAAAUGGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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