Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16256 | 3' | -47 | NC_004084.1 | + | 41767 | 0.66 | 0.993666 |
Target: 5'- cGAGGACCUCccggacGACGUCgaCGAcgaGGg -3' miRNA: 3'- -UUUCUGGAGaaa---CUGCAGaaGCUa--CC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 51730 | 0.66 | 0.991363 |
Target: 5'- --uGACCUCgagaugGACGUCgUCGAg-- -3' miRNA: 3'- uuuCUGGAGaaa---CUGCAGaAGCUacc -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 30594 | 0.66 | 0.991363 |
Target: 5'- --uGACCUCgcgGACGcCgcggUCGAUGu -3' miRNA: 3'- uuuCUGGAGaaaCUGCaGa---AGCUACc -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 18091 | 0.66 | 0.991363 |
Target: 5'- cGAGGACgaUCUcgGACGUCaggUCGAggucgGGg -3' miRNA: 3'- -UUUCUGg-AGAaaCUGCAGa--AGCUa----CC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 29571 | 0.66 | 0.989988 |
Target: 5'- uGAGGGCCaUCgccgggugGGCGUCguucgcgcgcagUUCGAUGGu -3' miRNA: 3'- -UUUCUGG-AGaaa-----CUGCAG------------AAGCUACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 56272 | 0.67 | 0.988282 |
Target: 5'- uGAAGuCCUCg--GuCGUCUggugaccUCGGUGGa -3' miRNA: 3'- -UUUCuGGAGaaaCuGCAGA-------AGCUACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 9337 | 0.67 | 0.986726 |
Target: 5'- cGAGACCUCUcccGAcCGUCgcacgUCGAcGGu -3' miRNA: 3'- uUUCUGGAGAaa-CU-GCAGa----AGCUaCC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 47009 | 0.67 | 0.984816 |
Target: 5'- cGAGuuCCUCgagGACGUCgcgaUCGAUGa -3' miRNA: 3'- uUUCu-GGAGaaaCUGCAGa---AGCUACc -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 52251 | 0.68 | 0.977825 |
Target: 5'- ---cGCCUUgcUGACGUCgccacCGAUGGa -3' miRNA: 3'- uuucUGGAGaaACUGCAGaa---GCUACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 17957 | 0.68 | 0.977825 |
Target: 5'- cGAGGCCgugugGACGUCgUCGGUGu -3' miRNA: 3'- uUUCUGGagaaaCUGCAGaAGCUACc -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 52700 | 0.68 | 0.975036 |
Target: 5'- cGAGGACCUCggcccgagcGACGUCcUCGAa-- -3' miRNA: 3'- -UUUCUGGAGaaa------CUGCAGaAGCUacc -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 18905 | 0.68 | 0.971999 |
Target: 5'- cGAGGACgUCgcgaacgaUGACGUCcUgGAUGGg -3' miRNA: 3'- -UUUCUGgAGaa------ACUGCAGaAgCUACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 28935 | 0.69 | 0.961308 |
Target: 5'- --cGGCCUCgggagcGCGUCgacagcgUCGAUGGg -3' miRNA: 3'- uuuCUGGAGaaac--UGCAGa------AGCUACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 21609 | 0.7 | 0.932149 |
Target: 5'- -uGGACCgUCUcggGACGaUCUUCGAcuUGGa -3' miRNA: 3'- uuUCUGG-AGAaa-CUGC-AGAAGCU--ACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 2828 | 0.7 | 0.926226 |
Target: 5'- --cGACCUCaaccucGGCGUCcgCGGUGGa -3' miRNA: 3'- uuuCUGGAGaaa---CUGCAGaaGCUACC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 15923 | 0.73 | 0.802638 |
Target: 5'- ---uGCgCUCUUUGACGUCUUUGA-GGu -3' miRNA: 3'- uuucUG-GAGAAACUGCAGAAGCUaCC- -5' |
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16256 | 3' | -47 | NC_004084.1 | + | 55550 | 1.09 | 0.007996 |
Target: 5'- cAAAGACCUCUUUGACGUCUUCGAUGGu -3' miRNA: 3'- -UUUCUGGAGAAACUGCAGAAGCUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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