Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16257 | 5' | -56.2 | NC_004084.1 | + | 35895 | 0.66 | 0.698528 |
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Target: 5'- uUCGGccGGaGGCGAUCGUCGUCGa-- -3' miRNA: 3'- gAGCUc-CCaCCGCUAGUAGCGGCaua -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 51154 | 0.66 | 0.687756 |
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Target: 5'- -aCGAGGa-GGCGAUCGagacggUCGCCGa-- -3' miRNA: 3'- gaGCUCCcaCCGCUAGU------AGCGGCaua -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 16107 | 0.66 | 0.687756 |
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Target: 5'- -cCGAcGGUcgucGCGAUCGUCGCCGa-- -3' miRNA: 3'- gaGCUcCCAc---CGCUAGUAGCGGCaua -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 2288 | 0.67 | 0.633318 |
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Target: 5'- aCUCGAGGGcaucgcgGGUcuGGUCGugcUCGCCGg-- -3' miRNA: 3'- -GAGCUCCCa------CCG--CUAGU---AGCGGCaua -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 21529 | 0.67 | 0.578839 |
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Target: 5'- cCUCGAGGaUGGCGAUCGcaucuucCGUCGg-- -3' miRNA: 3'- -GAGCUCCcACCGCUAGUa------GCGGCaua -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 20853 | 0.69 | 0.484085 |
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Target: 5'- -cCGAGGGUGGCacGUCGaccgCGCCGg-- -3' miRNA: 3'- gaGCUCCCACCGc-UAGUa---GCGGCaua -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 13212 | 0.71 | 0.387506 |
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Target: 5'- uUCGAGGGacugaucUGGaaggcagccCGAUCGUUGCCGUAc -3' miRNA: 3'- gAGCUCCC-------ACC---------GCUAGUAGCGGCAUa -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 21478 | 0.76 | 0.177746 |
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Target: 5'- cCUCGAGGGcGGCGAUCGccucgguugCGUCGUAg -3' miRNA: 3'- -GAGCUCCCaCCGCUAGUa--------GCGGCAUa -5' |
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| 16257 | 5' | -56.2 | NC_004084.1 | + | 55374 | 1.05 | 0.001587 |
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Target: 5'- cCUCGAGGGUGGCGAUCAUCGCCGUAUu -3' miRNA: 3'- -GAGCUCCCACCGCUAGUAGCGGCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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