Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16258 | 3' | -53.5 | NC_004084.1 | + | 41223 | 0.66 | 0.827705 |
Target: 5'- --aCGCCGUaCUUCACCaGGGUcu-ACGa -3' miRNA: 3'- gagGCGGUA-GAAGUGG-CCCAacuUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 47970 | 0.66 | 0.827705 |
Target: 5'- gUCCGCUcgaGUaCUUCugCGGGaUGcACGa -3' miRNA: 3'- gAGGCGG---UA-GAAGugGCCCaACuUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 28842 | 0.66 | 0.80913 |
Target: 5'- gUUCGCCGUCUcggUCACgaaccucgaaCGGG-UGGGCGu -3' miRNA: 3'- gAGGCGGUAGA---AGUG----------GCCCaACUUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 27030 | 0.66 | 0.80913 |
Target: 5'- -aCCGgC--CUUCGCCGGGcUGAACu -3' miRNA: 3'- gaGGCgGuaGAAGUGGCCCaACUUGc -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 35756 | 0.67 | 0.803408 |
Target: 5'- cCUCUGCCAUCUcgaucccguagagguUCgagccggaggcGCCGGGaaGGACGc -3' miRNA: 3'- -GAGGCGGUAGA---------------AG-----------UGGCCCaaCUUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 56711 | 0.68 | 0.728245 |
Target: 5'- -gCCGCCAUCcucCGCUGGGacucGGACGu -3' miRNA: 3'- gaGGCGGUAGaa-GUGGCCCaa--CUUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 33186 | 0.69 | 0.685119 |
Target: 5'- gUUCGCCcccgauuUUUUCACCGGGUUaGAcCGg -3' miRNA: 3'- gAGGCGGu------AGAAGUGGCCCAA-CUuGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 54536 | 0.69 | 0.641174 |
Target: 5'- uUCCGCUugaagaugaGUUcgUUCGCgGGGUUGGACa -3' miRNA: 3'- gAGGCGG---------UAG--AAGUGgCCCAACUUGc -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 41033 | 0.69 | 0.641174 |
Target: 5'- aCUCUGCCGgcgagaUCcUCACCGGcg-GAACGg -3' miRNA: 3'- -GAGGCGGU------AGaAGUGGCCcaaCUUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 52447 | 0.69 | 0.641174 |
Target: 5'- aCUCCGUCcUCcggCGCUGGGUUGuACa -3' miRNA: 3'- -GAGGCGGuAGaa-GUGGCCCAACuUGc -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 29626 | 0.72 | 0.510867 |
Target: 5'- -cCCGCUcgCcugCGCgGGGUUGAACGu -3' miRNA: 3'- gaGGCGGuaGaa-GUGgCCCAACUUGC- -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 15614 | 0.75 | 0.357606 |
Target: 5'- -aCCGgCGUC--CACCGGGUUGAACu -3' miRNA: 3'- gaGGCgGUAGaaGUGGCCCAACUUGc -5' |
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16258 | 3' | -53.5 | NC_004084.1 | + | 55046 | 1.1 | 0.001527 |
Target: 5'- gCUCCGCCAUCUUCACCGGGUUGAACGg -3' miRNA: 3'- -GAGGCGGUAGAAGUGGCCCAACUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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