Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 16259 | 3' | -55.4 | NC_004084.1 | + | 10123 | 0.72 | 0.425985 |
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Target: 5'- uCGACGCaCUCCggguuccgcaguUCGUCGGUucccuccUCCGGCUc -3' miRNA: 3'- uGCUGCG-GAGG------------AGCAGCUA-------AGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 1619 | 0.73 | 0.390573 |
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Target: 5'- uCGAUGaaCUCCUCGagGAggCCGACCg -3' miRNA: 3'- uGCUGCg-GAGGAGCagCUaaGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 31132 | 0.74 | 0.348081 |
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Target: 5'- aGCGGCGCCgcgaUCGUcagCGGUUCgGACCg -3' miRNA: 3'- -UGCUGCGGagg-AGCA---GCUAAGgCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 13558 | 0.75 | 0.280202 |
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Target: 5'- aGCGACGuCCUCCUggaaGUCGcgggccgaUCCGACCu -3' miRNA: 3'- -UGCUGC-GGAGGAg---CAGCua------AGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 37076 | 0.77 | 0.229078 |
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Target: 5'- uCGAgGuCCUCCUCGUCaGUaCCGACCu -3' miRNA: 3'- uGCUgC-GGAGGAGCAGcUAaGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 54188 | 1.12 | 0.000816 |
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Target: 5'- aACGACGCCUCCUCGUCGAUUCCGACCg -3' miRNA: 3'- -UGCUGCGGAGGAGCAGCUAAGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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