Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16259 | 3' | -55.4 | NC_004084.1 | + | 17332 | 0.66 | 0.778098 |
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Target: 5'- gGCGACgGCCcgcaucgUCUCGUCGAcguacucgCgGACCg -3' miRNA: 3'- -UGCUG-CGGa------GGAGCAGCUaa------GgCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 28135 | 0.66 | 0.778098 |
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Target: 5'- aAUGACGuCCUCgUC-UCGAUacUUCGGCUg -3' miRNA: 3'- -UGCUGC-GGAGgAGcAGCUA--AGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 3180 | 0.66 | 0.778098 |
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Target: 5'- -gGAUcaCCUUgUCGUCGGUUCCGAg- -3' miRNA: 3'- ugCUGc-GGAGgAGCAGCUAAGGCUgg -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 48525 | 0.66 | 0.778098 |
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Target: 5'- gACGuACGCCUUCaCGucaUCGGUcgCCGACUc -3' miRNA: 3'- -UGC-UGCGGAGGaGC---AGCUAa-GGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 10387 | 0.66 | 0.787799 |
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Target: 5'- uCGACGUCgagCUCGUCGucgUCCaggucgucGACCa -3' miRNA: 3'- uGCUGCGGag-GAGCAGCua-AGG--------CUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 16388 | 0.66 | 0.787799 |
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Target: 5'- gACGAUGUCgaccgCCUCGcUCGcga-CGGCCa -3' miRNA: 3'- -UGCUGCGGa----GGAGC-AGCuaagGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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