Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16259 | 3' | -55.4 | NC_004084.1 | + | 56120 | 0.67 | 0.696181 |
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Target: 5'- gACGucguGCGCCgcagUgUCGUCGGcgUCGACCa -3' miRNA: 3'- -UGC----UGCGGa---GgAGCAGCUaaGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 11854 | 0.67 | 0.696181 |
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Target: 5'- cGCGAUGCCga--UGcCGAgUCCGACCa -3' miRNA: 3'- -UGCUGCGGaggaGCaGCUaAGGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 16034 | 0.67 | 0.702524 |
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Target: 5'- cGCGG-GCCUCCUCGagGAUgcguugcucccgcUCGGCCu -3' miRNA: 3'- -UGCUgCGGAGGAGCagCUAa------------GGCUGG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 40891 | 0.67 | 0.70674 |
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Target: 5'- gUGACGCCUUCaacaUCGAUaUCCGACg -3' miRNA: 3'- uGCUGCGGAGGagc-AGCUA-AGGCUGg -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 19199 | 0.67 | 0.727638 |
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Target: 5'- gACGACGUCUCCgacagaGAUgucgCCGugCg -3' miRNA: 3'- -UGCUGCGGAGGagcag-CUAa---GGCugG- -5' |
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| 16259 | 3' | -55.4 | NC_004084.1 | + | 10387 | 0.66 | 0.787799 |
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Target: 5'- uCGACGUCgagCUCGUCGucgUCCaggucgucGACCa -3' miRNA: 3'- uGCUGCGGag-GAGCAGCua-AGG--------CUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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