Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16260 | 3' | -60.9 | NC_004084.1 | + | 50462 | 0.66 | 0.465 |
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Target: 5'- cCUGCggUCG-GCGGUGUCGuucgccgauguuGaGGGUCGg -3' miRNA: 3'- aGACG--AGCuCGCCACGGCu-----------C-CCCAGC- -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 49643 | 0.66 | 0.438617 |
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Target: 5'- cCUGCUCGAGC--UGUgGGcGGuGGUCGa -3' miRNA: 3'- aGACGAGCUCGccACGgCU-CC-CCAGC- -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 18549 | 0.66 | 0.438617 |
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Target: 5'- aUCgaaCUCGGGUGG-GCCGAcGGGGagaUCGc -3' miRNA: 3'- -AGac-GAGCUCGCCaCGGCU-CCCC---AGC- -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 45757 | 0.67 | 0.411325 |
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Target: 5'- gUCcGCUCGAuCGGgacGCCGAuGGaGGUCa -3' miRNA: 3'- -AGaCGAGCUcGCCa--CGGCU-CC-CCAGc -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 9133 | 0.67 | 0.36826 |
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Target: 5'- aUCgGCguccgUCGGGCGGaaGUCGAGGGGUa- -3' miRNA: 3'- -AGaCG-----AGCUCGCCa-CGGCUCCCCAgc -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 14289 | 0.68 | 0.320812 |
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Target: 5'- -aUGCUCGAGUGGUGUgGucuGGGaGUgGu -3' miRNA: 3'- agACGAGCUCGCCACGgCu--CCC-CAgC- -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 42011 | 0.72 | 0.186331 |
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Target: 5'- cUUUGCUCGAGUGGccgucGCgagCGAGGcGGUCGa -3' miRNA: 3'- -AGACGAGCUCGCCa----CG---GCUCC-CCAGC- -5' |
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| 16260 | 3' | -60.9 | NC_004084.1 | + | 52864 | 1.08 | 0.000404 |
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Target: 5'- aUCUGCUCGAGCGGUGCCGAGGGGUCGc -3' miRNA: 3'- -AGACGAGCUCGCCACGGCUCCCCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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