Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16260 | 5' | -56 | NC_004084.1 | + | 17839 | 0.66 | 0.700371 |
Target: 5'- -cCGAGUUcgauggugaCCUCGUCGCCCGCa---- -3' miRNA: 3'- caGCUCAA---------GGAGCGGUGGGUGauccu -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 46103 | 0.66 | 0.689616 |
Target: 5'- -cCGAcGUUCUUCGagaCCGuuucuCCCGCUGGGAg -3' miRNA: 3'- caGCU-CAAGGAGC---GGU-----GGGUGAUCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 29516 | 0.66 | 0.689616 |
Target: 5'- cUCGGGcacacccgCUUCGCCACCCGC-GGcGAg -3' miRNA: 3'- cAGCUCaa------GGAGCGGUGGGUGaUC-CU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 5012 | 0.66 | 0.689616 |
Target: 5'- -aCGAGgUCCUCgaggaguugguaGCCGCCCGacgAGGGc -3' miRNA: 3'- caGCUCaAGGAG------------CGGUGGGUga-UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 35143 | 0.66 | 0.667955 |
Target: 5'- cGUCGAGgUCCUCGggguaCAuCCCAaagAGGAc -3' miRNA: 3'- -CAGCUCaAGGAGCg----GU-GGGUga-UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 26084 | 0.66 | 0.667955 |
Target: 5'- cUCGuGcUCUUCGCCugC-GCUGGGAu -3' miRNA: 3'- cAGCuCaAGGAGCGGugGgUGAUCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 8355 | 0.66 | 0.667955 |
Target: 5'- uUCGAGagcaaUCGCCACCCGgUgaGGGAc -3' miRNA: 3'- cAGCUCaagg-AGCGGUGGGUgA--UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 22350 | 0.66 | 0.65707 |
Target: 5'- cGUCGcGgUCCUCGUCGuaucCCCACcAGGGu -3' miRNA: 3'- -CAGCuCaAGGAGCGGU----GGGUGaUCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 38692 | 0.67 | 0.634148 |
Target: 5'- -aCGAGgagUCCUCGCCACCaccguCguagucgaagucgUGGGAg -3' miRNA: 3'- caGCUCa--AGGAGCGGUGGgu---G-------------AUCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 35675 | 0.68 | 0.580781 |
Target: 5'- cGUCGAugUCCUCGUa--CCGCUGGGc -3' miRNA: 3'- -CAGCUcaAGGAGCGgugGGUGAUCCu -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 8646 | 0.68 | 0.565675 |
Target: 5'- -cCGAGgcuguagUCCUCGCCgagggucugggcgACCCACUcgagcaccucgaagAGGAg -3' miRNA: 3'- caGCUCa------AGGAGCGG-------------UGGGUGA--------------UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 32623 | 0.69 | 0.516876 |
Target: 5'- cUCGAcgUCgCUCGCCACCUGgaGGGAu -3' miRNA: 3'- cAGCUcaAG-GAGCGGUGGGUgaUCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 47654 | 0.69 | 0.496179 |
Target: 5'- cGUCGAGgucggCgaCGCC-CCCACUGGcGAg -3' miRNA: 3'- -CAGCUCaa---GgaGCGGuGGGUGAUC-CU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 34278 | 0.69 | 0.496179 |
Target: 5'- uUCGAGgauagUCCUugcgcccggagaCGCCGCCCAaagagAGGAa -3' miRNA: 3'- cAGCUCa----AGGA------------GCGGUGGGUga---UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 2214 | 0.72 | 0.355396 |
Target: 5'- aUCGAugUCCUCGCCGCUCGCUucGGc -3' miRNA: 3'- cAGCUcaAGGAGCGGUGGGUGAu-CCu -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 18806 | 0.73 | 0.322901 |
Target: 5'- cGUCGAGgaCgUCGCCGgCCACguccAGGAg -3' miRNA: 3'- -CAGCUCaaGgAGCGGUgGGUGa---UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 8155 | 0.77 | 0.164873 |
Target: 5'- cGUCGAGgaCCUCGCCGCCaacgGCgacgAGGAc -3' miRNA: 3'- -CAGCUCaaGGAGCGGUGGg---UGa---UCCU- -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 53060 | 0.81 | 0.097407 |
Target: 5'- cGUCGAGaucgucccagUCCUCGCCGCCCGCUcGGc -3' miRNA: 3'- -CAGCUCa---------AGGAGCGGUGGGUGAuCCu -5' |
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16260 | 5' | -56 | NC_004084.1 | + | 52897 | 1.09 | 0.000968 |
Target: 5'- gGUCGAGUUCCUCGCCACCCACUAGGAa -3' miRNA: 3'- -CAGCUCAAGGAGCGGUGGGUGAUCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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