Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16261 | 3' | -58.2 | NC_004084.1 | + | 54055 | 0.66 | 0.623112 |
Target: 5'- aCGgUUGGACGCgCGaCGaGGAgacGGGCGAg -3' miRNA: 3'- -GCgGACCUGCG-GCaGC-CCUa--UCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 6447 | 0.66 | 0.623112 |
Target: 5'- aCGCCcacUGGuCGCCGUggagCGGGAUguccuGGACc- -3' miRNA: 3'- -GCGG---ACCuGCGGCA----GCCCUA-----UCUGcu -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 38365 | 0.66 | 0.61249 |
Target: 5'- uGCCU--ACGCCGgaucgagcgCGGGA-AGGCGAc -3' miRNA: 3'- gCGGAccUGCGGCa--------GCCCUaUCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 58271 | 0.66 | 0.601884 |
Target: 5'- -aUCUGGAUgGCC-UCGGGGUGGGCc- -3' miRNA: 3'- gcGGACCUG-CGGcAGCCCUAUCUGcu -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 1130 | 0.66 | 0.601884 |
Target: 5'- gGuCCUGGAUGgCG-CGGuuGUAGACGAg -3' miRNA: 3'- gC-GGACCUGCgGCaGCCc-UAUCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 29072 | 0.66 | 0.591302 |
Target: 5'- cCGCCcgcucggGGGCGUCGUCGccgcGGUAGAUGc -3' miRNA: 3'- -GCGGa------CCUGCGGCAGCc---CUAUCUGCu -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 40915 | 0.66 | 0.581806 |
Target: 5'- gGCCUGGACGaggagcagcuacCGGGcUGGACGGa -3' miRNA: 3'- gCGGACCUGCggca--------GCCCuAUCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 39709 | 0.66 | 0.580752 |
Target: 5'- cCGCCaGugacggcgacGACGCCGUCGacgaGGAU-GACGAg -3' miRNA: 3'- -GCGGaC----------CUGCGGCAGC----CCUAuCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 6874 | 0.67 | 0.569194 |
Target: 5'- aGCCggucgUGGAUGCCGagguacgUCGGGAUc-GCGAc -3' miRNA: 3'- gCGG-----ACCUGCGGC-------AGCCCUAucUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 23992 | 0.67 | 0.518534 |
Target: 5'- aGCgaUGGAUGCgGUUGGGGgcucccAGACGGc -3' miRNA: 3'- gCGg-ACCUGCGgCAGCCCUa-----UCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 11030 | 0.67 | 0.518534 |
Target: 5'- gCGcCCUGGAgcuCGCCGUCGacGAcgcGGACGAa -3' miRNA: 3'- -GC-GGACCU---GCGGCAGCc-CUa--UCUGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 52047 | 0.68 | 0.468787 |
Target: 5'- gGUCU--ACGgCGUCGGGAUGGACu- -3' miRNA: 3'- gCGGAccUGCgGCAGCCCUAUCUGcu -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 21075 | 0.74 | 0.200314 |
Target: 5'- uCGagCUGGGCGUCGUCGGGAUcGcCGAa -3' miRNA: 3'- -GCg-GACCUGCGGCAGCCCUAuCuGCU- -5' |
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16261 | 3' | -58.2 | NC_004084.1 | + | 52475 | 1.09 | 0.000711 |
Target: 5'- uCGCCUGGACGCCGUCGGGAUAGACGAa -3' miRNA: 3'- -GCGGACCUGCGGCAGCCCUAUCUGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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