Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16261 | 5' | -55.6 | NC_004084.1 | + | 29754 | 0.66 | 0.753025 |
Target: 5'- ---cGUUgAGCUCGcUGCGGCGgUAGGu -3' miRNA: 3'- cuacCAGgUCGAGC-AUGCCGCaGUCC- -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 9698 | 0.66 | 0.732373 |
Target: 5'- aGGUGGUCCuugcuGCUCGaaaGGaCGUCGccGGa -3' miRNA: 3'- -CUACCAGGu----CGAGCaugCC-GCAGU--CC- -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 53041 | 0.66 | 0.732373 |
Target: 5'- ---aGUCCGGCUgGcACGGCGUCc-- -3' miRNA: 3'- cuacCAGGUCGAgCaUGCCGCAGucc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 39398 | 0.67 | 0.689981 |
Target: 5'- ---aGUCCAGUUCGagcuCGGCGUCGu- -3' miRNA: 3'- cuacCAGGUCGAGCau--GCCGCAGUcc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 25461 | 0.68 | 0.624964 |
Target: 5'- cGUGGcUUCgaGGUUCGcggGCGGCGUCAGa -3' miRNA: 3'- cUACC-AGG--UCGAGCa--UGCCGCAGUCc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 52595 | 0.68 | 0.624964 |
Target: 5'- cGUGGUCCGGUgggucgaggcagUCGacacgccCGGCGUCGGa -3' miRNA: 3'- cUACCAGGUCG------------AGCau-----GCCGCAGUCc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 45964 | 0.68 | 0.603237 |
Target: 5'- cGAUGuGUCCgagucGGCUCcUGCGGuCGUCAcGGc -3' miRNA: 3'- -CUAC-CAGG-----UCGAGcAUGCC-GCAGU-CC- -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 49678 | 0.69 | 0.570848 |
Target: 5'- gGGUGGUCCG--UCGUcuCGGUGUCGGa -3' miRNA: 3'- -CUACCAGGUcgAGCAu-GCCGCAGUCc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 45030 | 0.7 | 0.477057 |
Target: 5'- --cGGUUCAGCUCGaACuGGCGcUCGGa -3' miRNA: 3'- cuaCCAGGUCGAGCaUG-CCGC-AGUCc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 7938 | 0.72 | 0.373921 |
Target: 5'- --cGGUCaguuGCUCGUGCGGCGUUc-- -3' miRNA: 3'- cuaCCAGgu--CGAGCAUGCCGCAGucc -5' |
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16261 | 5' | -55.6 | NC_004084.1 | + | 52510 | 1.1 | 0.000861 |
Target: 5'- uGAUGGUCCAGCUCGUACGGCGUCAGGa -3' miRNA: 3'- -CUACCAGGUCGAGCAUGCCGCAGUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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